{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T16:04:12Z","timestamp":1785341052770,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Urolithiasis, a condition characterized by the formation of calcium oxalate stones in the urinary tract, remains a significant clinical challenge due to the limited availability of effective preventive therapies. This study investigated the antiurolithic potential of different preparations from Eugenia mattosii, a plant rich in phenolic constituents. Calcium oxalate (CaOx) precipitation was induced in rat urine samples. Potassium citrate (10\u00a0mg\/mL) was used as the positive control, while the crude methanolic extract (CME), chloroform fraction (CLF), ethyl acetate fraction (EAF), pinostrobin (PIN), and cryptostrobin (CRY) were tested at concentrations ranging from 0.01 to 1\u00a0mg\/mL. CME and EAF increased CaOx crystal formation, whereas CLF, PIN, and CRY significantly reduced crystal formation at 0.01\u00a0mg\/mL. In silico ADMET analysis supported favorable pharmacokinetic and safety profiles for PIN and CRY. Molecular docking revealed strong interactions with MMP\u20109, suggesting a potential inhibitory effect that may reduce tissue injury and crystal nucleation. Overall, CLF and its flavonoids demonstrate promising antiurolithic activity.<\/jats:p>","DOI":"10.1002\/cbdv.202503734","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T19:27:38Z","timestamp":1776454058000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Silico and In Vitro Antiurolithic Effect of Extracts, Fractions, and Isolated Compounds From\n                    <i>Eugenia mattosii<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Bianca Let\u00edcia","family":"Maci\u00e9l","sequence":"first","affiliation":[{"name":"Nutrition Department Universidade do Vale do Itaja\u00ed\u00a0UNIVALI  Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luma Da Silva","family":"Portella","sequence":"additional","affiliation":[{"name":"Nutrition Department Universidade do Vale do Itaja\u00ed\u00a0UNIVALI  Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camile Cecconi","family":"Cechinel\u2010Zanchett","sequence":"additional","affiliation":[{"name":"Nutrition Department Universidade do Vale do Itaja\u00ed\u00a0UNIVALI  Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anelize","family":"Dada","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Universidade Do Vale do Itaja\u00ed\u00a0UNIVALI  Itaja\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita de C\u00e1ssia Vilhena","family":"da Silva","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Universidade Do Vale do Itaja\u00ed\u00a0UNIVALI  Itaja\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anelise Fel\u00edcio","family":"Macarini","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Universidade Do Vale do Itaja\u00ed\u00a0UNIVALI  Itaja\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdir","family":"Cechinel Filho","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Universidade Do Vale do Itaja\u00ed\u00a0UNIVALI  Itaja\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5251-5642","authenticated-orcid":false,"given":"Priscila","family":"de Souza","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Universidade Do Vale do Itaja\u00ed\u00a0UNIVALI  Itaja\u00ed Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovana","family":"Vechi","sequence":"additional","affiliation":[{"name":"Nutrition Department Universidade do Vale do Itaja\u00ed\u00a0UNIVALI  Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/np200906s"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01285"},{"key":"e_1_2_10_4_1","unstructured":"M. J. L.Danta T. D. A.Ara\u00fajo A. B.daSilva andJ. B. P.deSouza \u201cCristais de oxalato em quebra\u2010pedra (Phyllanthus amarus) e o uso irracional de plantas no tratamento da urolit\u00edase \u201d (2019)."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3068365"},{"key":"e_1_2_10_6_1","first-page":"496","article-title":"Calcium Oxalate Urolithiasis","volume":"31","author":"Gisselman K.","year":"2009","journal-title":"Compend Contin Educ Vet Compend Contin Educ Vet"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5216\/ref.v5i2.5148","article-title":"Pharmacognostic Characterization of the Leaves of Eugenia Uniflora L. (Myrtaceae)","volume":"5","author":"Fiuza T. 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Herbario Barbosa Rodrigues itaja\u00ed Santa Catarina Brazil \u201d (1969)."},{"key":"e_1_2_10_12_1","unstructured":"M.Sobral E.Lucas L.Landrum et\u00a0al. \u201cPlantas da Floresta Atl\u00e2ntica \u201dRio de Janeiro:Jardim Bot\u00e2nico do Rio de Janeiro (2009)."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1901400111"},{"key":"e_1_2_10_14_1","first-page":"1","article-title":"Phenolic Profile by HPLC\u2010ESI\u2010MS\/MS of Six Brazilian Eugenia Species and Their Potential as Cholinesterase Inhibitors","volume":"1","author":"Tenfen A.","year":"2019","journal-title":"Natural Product Research"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-019-1973-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.11.039"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.389"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2018.1439880"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.5530\/pj.2018.5.148"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpp\/rgaa071"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijp.IJP_92_18"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116157"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.982419"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/292953"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00240\u2010018\u20101096\u20100"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b01420"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00037.2012"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503734","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.202503734","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.202503734","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:49:41Z","timestamp":1785340181000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.202503734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/cbdv.202503734"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.202503734","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03734"}}