{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:01:48Z","timestamp":1785484908275,"version":"3.56.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Benzylisoquinoline alkaloids (BIAs) are a diverse group of plant secondary metabolites with significant pharmacological activities, where methylation reactions catalyzed by methyltransferases play crucial roles in structural diversification and bioactivity modulation. In this study, we identified and functionally characterized two key methyltransferases,\n                    <jats:italic>Sinomenium acutum<\/jats:italic>\n                    6\u2010O\u2010methyltransferase (Sa6OMT) and\n                    <jats:italic>S. acutum<\/jats:italic>\n                    coclaurine\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyltransferase (SaCNMT), involved in BIA biosynthesis in\n                    <jats:italic>S. acutum<\/jats:italic>\n                    , a traditional Chinese medicinal plant with anti\u2010rheumatoid arthritis properties. Candidate genes, including\n                    <jats:italic>SaOMT3<\/jats:italic>\n                    and\n                    <jats:italic>SaNMT4<\/jats:italic>\n                    , were selected through transcriptome analysis of different tissues combined with phylogenetic analysis and expression pattern evaluation. Functional validation demonstrated that SaOMT3 encodes a 6OMT that catalyzes the conversion of (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010norcoclaurine to (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010coclaurine, while SaNMT4 encodes a CNMT that mediates the\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylation of (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010coclaurine to (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylcoclaurine. Biochemical characterization revealed optimal activity at 40\u00b0C for both enzymes, with distinct optimal pH levels (8.5 for Sa6OMT and 8.0 for SaCNMT) and higher catalytic efficiency for SaCNMT. This study provides valuable insights into the molecular mechanisms underlying BIA biosynthesis in\n                    <jats:italic>S. acutum<\/jats:italic>\n                    and enhances our understanding of plant specialized metabolism.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503736","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T18:19:41Z","timestamp":1769019581000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Characterization of Two Methyltransferases Involved in Benzylisoquinoline Alkaloid Biosynthesis in\n                    <i>Sinomenium acutum<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Yuxin","family":"Qi","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine School of Pharmaceutical Sciences Hunan University of Medicine  Huaihua 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