{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:05:43Z","timestamp":1785330343985,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, a strategy for determining the optimal harvesting time of medicinal plants was proposed and implemented, using the antitumor herb\n                    <jats:italic>Selaginella doederleinii<\/jats:italic>\n                    Hieron (\n                    <jats:italic>S. doederleinii<\/jats:italic>\n                    ) as a case study, based on HPLC fingerprinting profiles and in vitro activity assays. A total of 12 batches of\n                    <jats:italic>S. doederleinii<\/jats:italic>\n                    samples were collected from a fixed geographic location from January to December. An HPLC\u2010based fingerprint was then established, followed by similarity analysis, principal component analysis (PCA), and partial least squares discriminant analysis (PLS\u2010DA) on the 80% ethanol extracts of these samples. Meanwhile, in vitro viability was assessed using Calcein\/PI cell viability assays and Western blotting analysis. By combining the chemometrics and biological activity results, it is inferred that the optimal harvesting time for\n                    <jats:italic>S. doederleinii<\/jats:italic>\n                    is in April and May. Furthermore, the following compounds were tentatively identified as quality control markers for this medicinal herb at the fixed geographic location: 2\u2033,3\u2033\u2010Dihydro\u20103\u2032,3\u2034\u2010biapigenin, 3\u2032,3\u2034\u2010binaringenin, Delicaflavone, 2,3\u2010Dihydrohinokiflavone, Chrysocauloflavone I, and 2\u2033,3\u2033\u2010Dihydro\u20103\u2032,3\u2034\u2010biapigenin methyl ether. This study provides a feasible strategy for selecting harvesting time of medicinal plants grown at a fixed geographic location.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503753","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:36:26Z","timestamp":1784129786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Optimal Harvesting Time for\n                    <i>Selaginella doederleinii<\/i>\n                    Hieron at a Fixed Location Using HPLC Fingerprinting and In Vitro Antiproliferation Assays"],"prefix":"10.1002","volume":"23","author":[{"given":"Huilin","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Pharmacy Fujian Medical University Union Hospital Fuzhou China"},{"name":"Department of Pharmaceutical Analysis School of Pharmacy Fujian Medical University Fuzhou China"},{"name":"Fujian Provincial Key Laboratory of Hepatic Drug Research  Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Fujian Medical University Union Hospital Fuzhou China"},{"name":"Department of Pharmaceutical Analysis School of Pharmacy Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Hu","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Hepatic Drug Research  Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis School of Pharmacy Fujian Medical University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Fujian Medical University Union Hospital Fuzhou 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