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Among these types, cutaneous melanoma is one of the most common types of skin cancer worldwide. Newer, non\u2010invasive, and more effective forms of treatment are urgently needed. Recently, the cancer membrane has emerged as a novel target for new anticancer drugs. Serine protease inhibitors are researched across all kingdoms; however, the Plantae kingdom is a more sustainable producer of bioactive molecules, including serine protease inhibitors. In this study, a Kunitz trypsin inhibitor (JTI) purified by RP\u2010HPLC from\n                    <jats:italic>Mimosa regnellii<\/jats:italic>\n                    seeds was sequenced, its inhibitory activity quantified, and its inhibition constant determined. It was then tested for its ability to induce cell death via the apoptotic pathway in B16\u2010F10 mouse melanoma cells. To evaluate its pro\u2010apoptotic capacity, the inhibitor's effects on reactive oxygen species release, calcium release, cell morphological changes, and the inhibition of angiogenic and migratory activities of rabbit endothelial cells (RaEC) were also assessed in vitro. Tests for toxicity against normal cells were performed, confirming that JTI acts on melanoma cells with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 0.65\u00a0\u00b5M. These results suggest that JTI has potential for use in melanoma treatment.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503760","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T12:20:47Z","timestamp":1776774047000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biochemical Characterization of a Kunitz\u2010Type Protease Inhibitor From\n                    <i>Mimosa regnellii<\/i>\n                    and Its Effects on Melanoma Cell Viability and Angiogenesis"],"prefix":"10.1002","volume":"23","author":[{"given":"Luciana Maria Ara\u00fajo","family":"Rab\u00ealo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio De Qu\u00edmica e Fun\u00e7\u00e3o De Prote\u00ednas Bioativas Centro de Bioci\u00eancias Departamento De Bioqu\u00edmica Universidade Federal do Rio Grande Do Norte  Natal Rio Grande do Norte 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