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Building on our previous studies of cytotoxic compounds from zoantharians of the genus\n                    <jats:italic>Palythoa<\/jats:italic>\n                    , we investigated the anticancer potential of bacteria associated with\n                    <jats:italic>Palythoa variabilis<\/jats:italic>\n                    . Ten culturable bacterial strains were isolated and screened for cytotoxic activity against metastatic prostate cancer cells (PC\u20103\/M) using the MTT assay. Among them, the crude extract of strain BRA\u2010035, identified as\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp., showed the most promising bioactivity profile and was selected for cytotoxicity\u2010guided fractionation. This approach enabled the isolation of piericidin A1 (\n                    <jats:bold>1<\/jats:bold>\n                    ) and the identification of two additional piericidin derivatives (\n                    <jats:bold>2<\/jats:bold>\n                    and\n                    <jats:bold>3<\/jats:bold>\n                    ). piericidin A1 exhibited strong and selective cytotoxicity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from picomolar to low nanomolar concentrations against ovarian (OVCAR), prostate (PC\u20103 and PC\u20103\/M), and colorectal (HCT\u2010116) tumor cell lines, while remaining inactive (IC\n                    <jats:sub>50<\/jats:sub>\n                    &gt; 12\u00a0\u00b5M) against HL\u201060 leukemia and B16\u2010F10 murine melanoma cells. Additional assays assessing cell proliferation and membrane integrity provided further insight into differential cellular sensitivity. Overall, these results demonstrate that\n                    <jats:italic>P. variabilis<\/jats:italic>\n                    \u2010associated bacteria are a valuable source of bioactive metabolites and reinforce the translational potential of piericidin A1 as a promising anticancer drug candidate.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.202503815","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T19:42:52Z","timestamp":1776714172000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anticancer Potential of Piericidin A1 and Derivatives Isolated From\n                    <i>Streptomyces<\/i>\n                    sp. Associated With\n                    <i>Palythoa variabilis<\/i>\n                    From Brazilian Reefs"],"prefix":"10.1002","volume":"23","author":[{"given":"Bianca Del B.","family":"Sahm","sequence":"first","affiliation":[{"name":"Departamento De Farmacologia Universidade De S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharine G. D.","family":"Flor\u00eancio","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa e Desenvolvimento de Medicamentos Departamento De Fisiologia e Farmacologia Universidade Federal do Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco C. L.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Departamento De Qu\u00edmica Org\u00e2nica e Inorg\u00e2nica Universidade Federal do Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana I. V.","family":"Maia","sequence":"additional","affiliation":[{"name":"Departamento De Qu\u00edmica Org\u00e2nica e Inorg\u00e2nica Universidade Federal do Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A. M.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Coordena\u00e7\u00e3o de Recursos Pesqueiros e Agroneg\u00f3cio Instituto Federal de Educa\u00e7\u00e3o Ci\u00eancia e Tecnologia do Par\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula C.","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Instituto Do Mar Universidade Federal de S\u00e3o Paulo  Santos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ot\u00edlia D. L.","family":"Pessoa","sequence":"additional","affiliation":[{"name":"Departamento De Qu\u00edmica Org\u00e2nica e Inorg\u00e2nica Universidade Federal do Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tito M. C.","family":"Lotufo","sequence":"additional","affiliation":[{"name":"Instituto Oceanogr\u00e1fico Universidade De S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia V.","family":"Costa\u2010Lotufo","sequence":"additional","affiliation":[{"name":"Departamento De Farmacologia Universidade De S\u00e3o Paulo  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9481-6702","authenticated-orcid":false,"given":"Diego V.","family":"Wilke","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa e Desenvolvimento de Medicamentos Departamento De Fisiologia e Farmacologia Universidade Federal do Cear\u00e1  Fortaleza Cear\u00e1 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