{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T16:00:40Z","timestamp":1785081640851,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Baichuan Baile (BCBL) is a novel herbal formula composed of three herbs and a food additive with proven antidepressant\u2010like effects. However, its unclear bioactive constituents hinder quality control. This study evaluated BCBL\u2010CP, a fraction extracted via ethanol precipitation and macroporous resin chromatography, mainly comprising coumarins (5\u2010hydroxyxanthotoxin, oxypeucedanin hydrate, byakangelicin) and phthalides (senkyunolides H and I). We rapidly assessed BCBL\u2010CP's efficacy using behavioral despair and 5\u2010hydroxytryptophan\u2010induced head\u2010twitch tests. Subsequently, therapeutic effects on a reserpine\u2010induced depression\u2010like mouse model were evaluated through behavioral assessments. Its effects on the monoaminergic system, cerebral pathology, brain\u2010derived neurotrophic factor (BDNF), and gut microbiota were detected through enzyme\u2010linked immunosorbent assay, hematoxylin and eosin staining, immunofluorescence, and 16S rDNA sequencing. Results showed BCBL\u2010CP exhibited significant antidepressant\u2010like activity in both acute behavioral screening and chronic reserpine\u2010induced depression studies. Mechanistically, BCBL\u2010CP selectively increased prefrontal norepinephrine (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05), attenuated neuronal damage\n                    <jats:italic>(p<\/jats:italic>\n                    &lt; 0.001), and showed a trend toward BDNF upregulation (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.083). Uniquely, it restored gut microbiota \u03b1\u2010diversity (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01), normalized \u03b2\u2010diversity, and modulated eight key bacterial taxa (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01 or\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). These findings confirm that these five compounds constitute the major characteristic bioactive constituents of BCBL, providing quality\u2010control markers for developing this prospective antidepressant.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71322","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:42:58Z","timestamp":1778654578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Coumarin\u2010 and Phthalide\u2010Rich Fraction From Baichuan Baile Formula Alleviates Reserpine\u2010Induced Depression\u2010Like Behaviors by Modulating the Monoaminergic System and Gut Microbiota"],"prefix":"10.1002","volume":"23","author":[{"given":"Chun\u2010Xue","family":"Gao","sequence":"first","affiliation":[{"name":"North China University of Science and Technology  Tangshan China"},{"name":"Academy of Military Medical Sciences  Beijing 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