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In the present study, a series of quinazolinone\u20101,3,4\u2010thiadiazole molecular hybrids were designed, synthesized and biologically evaluated for their antidiabetic potential. The full structural elucidation of each molecular hybrid was carried out using 1D\/2D NMR spectroscopy, FT\u2010IR and high\u2010resolution mass spectrometry (HRMS). All the synthesized compounds, including intermediates (\n                    <jats:bold>2a\u2010c<\/jats:bold>\n                    ,\n                    <jats:bold>3a\u2010g<\/jats:bold>\n                    ) and final hybrids (\n                    <jats:bold>5a\u2010u<\/jats:bold>\n                    ), were tested for \u03b1\u2010amylase inhibition. Compounds\n                    <jats:bold>3b<\/jats:bold>\n                    and\n                    <jats:bold>5t<\/jats:bold>\n                    exhibited approximately twofold higher potency than the standard drug acarbose. Cytotoxicity evaluation against hepatocellular carcinoma (HepG2) cells revealed no significant cytotoxicity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values exceeding 100 \u00b5M. Furthermore, molecular docking and simulation studies were conducted to analyse the ligand\u2010receptor interactions, while ADME\/T predictions suggested that the most potent compounds possess favourable drug\u2010like properties and could serve as promising lead candidates for further development.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71358","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:50:46Z","timestamp":1779688246000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antidiabetic and Cytotoxicity Studies of Novel Quinazolinone\u20131,3,4\u2010Thiadiazole Molecular Hybrids"],"prefix":"10.1002","volume":"23","author":[{"given":"Prishani","family":"Kisten","sequence":"first","affiliation":[{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pule","family":"Seboletswe","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4896-9553","authenticated-orcid":false,"given":"Gobind","family":"kumar","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nontobeko","family":"Gcabashe","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Manhas","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Ankit","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9918-5409","authenticated-orcid":false,"given":"Almahi","family":"Idris","sequence":"additional","affiliation":[{"name":"Discipline of Biological Sciences School of Agriculture &amp; Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-9491","authenticated-orcid":false,"given":"Md. 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