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The results revealed several potent inhibitors, with the most active compound, 1\u2010(4\u2010(dimethylamino)benzylidene)thiosemicarbazide (\n                    <jats:bold>6g<\/jats:bold>\n                    ), exhibiting approximately two\u2010fold greater potency compared to the reference drug acarbose. Structure\u2010activity relationship analysis indicated that thiosemicarbazones derived from benzaldehydes and ketones (\n                    <jats:bold>6a\u20136k<\/jats:bold>\n                    ,\n                    <jats:bold>7a\u2013e<\/jats:bold>\n                    ) contributed predominantly to the observed activity, outperforming analogues incorporating alternative pharmacophoric scaffolds such as isatin and furan (\n                    <jats:bold>8a\u2013b<\/jats:bold>\n                    and\n                    <jats:bold>9a\u2013d<\/jats:bold>\n                    ). In addition to enzyme inhibition, several compounds demonstrated notable antioxidant activity across multiple in vitro assays, including nitric oxide (NO), DPPH radical scavenging, and ferric reducing antioxidant power (FRAP) assays. Molecular docking studies of representative compounds within the catalytic site of \u03b1\u2010amylase highlighted the critical role of hydrogen bonding and \u03c0\u2010\u03c0 stacking interactions in stabilizing ligand\u2010enzyme complexes. Furthermore, in silico pharmacokinetic and drug\u2010likeness evaluations revealed that all synthesized compounds exhibited favorable profiles, in contrast to acarbose, which showed multiple violations of standard drug\u2010likeness criteria.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71362","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:00:09Z","timestamp":1779688809000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antidiabetic Evaluation of Thiosemicarbazone Analogs Through \u03b1\u2010Amylase Inhibition Complemented With Computational Studies"],"prefix":"10.1002","volume":"23","author":[{"given":"Neha","family":"Manhas","sequence":"first","affiliation":[{"name":"Department of Chemistry Durban University of Technology  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4896-9553","authenticated-orcid":false,"given":"Gobind","family":"Kumar","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry, School of Agriculture and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Ankit","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry, School of Agriculture and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5354-9545","authenticated-orcid":false,"given":"Nagaraju","family":"Kerru","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry, School of Agriculture and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pule","family":"Seboletswe","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry, School of Agriculture and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nontobeko","family":"Gcabashe","sequence":"additional","affiliation":[{"name":"Discipline of Chemistry, School of Agriculture and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9918-5409","authenticated-orcid":false,"given":"Almahi","family":"Idris","sequence":"additional","affiliation":[{"name":"Discipline of Biological Sciences, School of Agriculture and Science University of KwaZulu\u2010Natal  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0309-9491","authenticated-orcid":false,"given":"Md. 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