{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T23:25:53Z","timestamp":1786317953073,"version":"build-2736575974"},"reference-count":91,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Diospyros sylvatica<\/jats:italic>\n                    Roxb. (Ebenaceae) is a tropical medicinal plant traditionally used for respiratory, inflammatory, cold, and digestive disorders due to its rich terpenoids, tannins, polyphenols, and naphthoquinones. This study investigated the in vitro antioxidant, in vivo hypoglycemic, and analgesic activities, as well as the in silico molecular interactions, of GC\u2010MS\/MS\u2010guided phytochemicals from the leaves. Antihyperglycemic activity was evaluated using the oral glucose tolerance test, and peripheral analgesic activity was assessed using the acetic acid\u2010induced writhing model in Swiss albino mice (\n                    <jats:italic>n<\/jats:italic>\n                    = 6 per group). At the same time, antioxidant potential was determined using the DPPH assay %). The ethyl acetate fraction exhibited the highest antioxidant activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 35.28\u00a0\u00b5g\/mL). At 400\u00a0mg\/kg, chloroform and ethyl acetate fractions significantly reduced blood glucose by 50.14% and 36.60% at 180 min compared with metformin (64.02%). The\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane fraction showed the strongest analgesic activity with 50.40% writhing inhibition compared to diclofenac sodium (69.77%). GC\u2010MS\/MS analysis identified 29 compounds, which were further subjected to molecular docking and ADMET analysis, revealing drug\u2010likeness and favorable binding affinities toward targeted receptors. Collectively, these findings demonstrate significant antioxidant, hypoglycemic, and analgesic potentials of\n                    <jats:italic>D. sylvatica<\/jats:italic>\n                    leaves supported by identified bioactive metabolites, warranting further extensive studies for clinical translation.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71364","type":"journal-article","created":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T18:21:42Z","timestamp":1780251702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An Integrated Pharmacological Evaluation and GC\u2010MS\/MS\u2010Guided Metabolite Profiling of\n                    <i>Diospyros sylvatica<\/i>\n                    Roxb. Leaves Revealing Analgesic, Antioxidant, and Antidiabetic Activities"],"prefix":"10.1002","volume":"23","author":[{"given":"Most. Sunzida","family":"Afrose","sequence":"first","affiliation":[{"name":"Department of Pharmacy Islamic University Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md.","family":"Shamim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Saiful","family":"Islam","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Research Division BCSIR Dhaka Laboratories Bangladesh Council of Scientific and Industrial Research (BCSIR) Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M. Yasin","family":"Shourav","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology Faculty of Pharmacy University of Dhaka Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Riaz","family":"Hosen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaikh Shahinur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Applied Nutrition and Food Technology Islamic University Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1831-2278","authenticated-orcid":false,"given":"Safaet","family":"Alam","sequence":"additional","affiliation":[{"name":"Chemical Research Division BCSIR Dhaka Laboratories Bangladesh Council of Scientific and Industrial Research (BCSIR) Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9247-809X","authenticated-orcid":false,"given":"Md. Rasul","family":"Karim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,31]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202501015"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-021-03290-6"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e27533"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202500553"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2025.120762"},{"key":"e_1_2_13_7_1","first-page":"33","volume-title":"Plants and Secondary Metabolites","author":"Sahu A. 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