{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:14:44Z","timestamp":1783368884675,"version":"3.54.6"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005283","name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fungal species of the genus\n                    <jats:italic>Candida<\/jats:italic>\n                    are commensal in immunocompetent individuals but may become pathogenic under immunosuppressive conditions. Likewise,\n                    <jats:italic>Pichia kudriavzevii<\/jats:italic>\n                    has emerged as an opportunistic yeast associated with nosocomial infections, especially in immunocompromised patients. The increasing resistance to conventional antifungal agents has stimulated the search for alternative therapies, including essential oils. This study investigated the chemical composition and antifungal activity of the essential oil from fresh leaves of\n                    <jats:italic>Myrcia spectabilis<\/jats:italic>\n                    (EOFLMS) against\n                    <jats:italic>Candida<\/jats:italic>\n                    spp. and\n                    <jats:italic>P. kudriavzevii<\/jats:italic>\n                    . The oil was obtained by hydrodistillation and analyzed by GC\u2013MS, revealing a predominance of sesquiterpenes, mainly germacrene B, germacrene D, bicyclogermacrene, \u03b1\u2010cadinol, cis\u2010cadin\u20104\u2010en\u20107\u2010ol, and cubeban\u201011\u2010ol. Antifungal activity was evaluated through fungal growth curves, IC\n                    <jats:sub>50<\/jats:sub>\n                    , Minimum fungicidal concentration (MFC), and pleomorphism assays. EOFLMS alone showed moderate activity, especially against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , reducing fungal growth by 99.25% (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a01215 \u00b5g\/mL). In combination with fluconazole, EOFLMS enhanced antifungal activity against\n                    <jats:italic>C. albicans<\/jats:italic>\n                    and\n                    <jats:italic>Candida tropicalis<\/jats:italic>\n                    , with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 22.91 and 12.38 \u00b5g\/mL, respectively. EOFLMS also inhibited hyphal and pseudohyphal formation, suggesting interference with fungal virulence factors.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71374","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T12:39:21Z","timestamp":1780144761000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Composition and Antifungal Evaluation of Essential Oil From Fresh Leaves of\n                    <i>Myrcia spectabilis<\/i>\n                    DC Against\n                    <i>Candida<\/i>\n                    spp. and\n                    <i>Pichia kudriavzevii<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Francildo dos Santos","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Everson da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department Federal University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanderlei do","family":"Amaral","sequence":"additional","affiliation":[{"name":"Department Federal University of Paran\u00e1  Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciene Ferreira de","family":"Lima","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro Ruan Silva de","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7175-4465","authenticated-orcid":false,"given":"Camila","family":"Confortin","sequence":"additional","affiliation":[{"name":"Universidade Federal do Paran\u00e1\u2010Brazil \u2010 Post\u2010Graduate Program in Sustainable Territorial Development  Matinhos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrielson Jos\u00e9 da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Audilene de","family":"Freitas","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joara N\u00e1lyda Pereira","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u00eds Gusm\u00e3o da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alicy Neres de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Juno Alencar","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Flaviana Bezerra","family":"Morais\u2010Braga","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6433-3168","authenticated-orcid":false,"given":"Antonia Thassya Lucas dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biological Science Regional University of Cariri  Crato Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14090876"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/diseases13040093"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ram.2022.08.003"},{"key":"e_1_2_10_5_1","first-page":"789","article-title":"Occurrence of Pathogenic Yeast Species in Artisanal Cheeses From Boyac\u00e1, Colombia, Including Fluconazole Resistant Isolates","volume":"13","author":"Quitian Z. A. S.","year":"2024","journal-title":"F1000Research"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofaf742"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40506\u2010025\u201000284\u20107"},{"key":"e_1_2_10_8_1","article-title":"Antifungal Treatment Strategies and Their Impact on Resistance Development in Clinical Settings","volume":"80","author":"Rhijn N. V.","year":"2025","journal-title":"Journal of Antimicrobial Chemotherapy"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/idr17040091"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.54751\/revistafoco.v17n9-113"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019752"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms14010241"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0269721"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.05.037"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/20420986221143266"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.34119\/bjhrv3n1\u2010007"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v11i6.28385"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14050503"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107168"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010024\u201003487\u2010y"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010025\u201004437\u20100"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2024.118435"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22071100"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26237259"},{"key":"e_1_2_10_25_1","unstructured":"Embrapa Territorial.Agricultura e preserva\u00e7\u00e3o ambiental: uma an\u00e1lise do cadastro ambiental rural. Campinas 2020. Dispon\u00edvel em: <www.embrapa.br\/car>. Acesso em: 7 mar.2024."},{"key":"e_1_2_10_26_1","unstructured":"M. F.Santos B. S.Amorim G. P.Burton et\u00a0al. \u201cMyrcia in Flora e Funga do Brasil. Jardim Bot\u00e2nico do Rio de Janeiro \u201d Dispon\u00edvel em: <https:\/\/floradobrasil.jbrj.gov.br\/FB10758>. Acesso em: 27 fev.2026."},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofac559"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29122720"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001\u20103765202220210820"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-023-03969-y"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25040783"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.55905\/cuadv16n6-049"},{"key":"e_1_2_10_33_1","unstructured":"W.Amaral \u201cProspec\u00e7\u00e3o de esp\u00e9cies arom\u00e1ticas silvestres dos Campos Gerais da Mata Atl\u00e2ntica do Paran\u00e1. (2015) \u201d (2015) Tese (Doutorado) do Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Agronomia Produ\u00e7\u00e3o Vegetal 137 f. Curitiba."},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X241309736"},{"issue":"11","key":"e_1_2_10_35_1","first-page":"41","article-title":"Chemical Characterization, Antioxidant and Antimicrobial Activities of the Leaf Essential Oil of Syzgium guineense (Willd.) DC. Var. Guineense (Myrtaceae) From Nigeria","volume":"10","author":"Okhale S. E.","year":"2018","journal-title":"International Journal of Pharmacognosy and Phytochemical Research"},{"key":"e_1_2_10_36_1","article-title":"Composi\u00e7\u00e3o qu\u00edmica, atividades antimicrobiana e larvicida de \u00f3leos essenciais de duas esp\u00e9cies de Syzygium do Vietn\u00e3","volume":"84","author":"Huong L. T.","year":"2023","journal-title":"Revista Brasileira de Biologia"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16129827"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.33448\/rsd\u2010v10i11.19354"},{"issue":"3","key":"e_1_2_10_39_1","article-title":"Composi\u00e7\u00e3o qu\u00edmica, atividade antimicrobiana e antioxidante do \u00f3leo essencial das folhas de Myrcia palustris DC. (MYRTACEAE)","volume":"10","author":"Santos C. V.","year":"2021","journal-title":"Pesquisa, Sociedade e Desenvolvimento"},{"key":"e_1_2_10_40_1","doi-asserted-by":"crossref","unstructured":"C. B.Santana J. G. L.Souza M. D. A.Coracini et\u00a0al. \u201cChemical composition of essential oil from Myrcia oblongata DC and potencial antimicrobial antioxidant and acaricidal activity against Dermanyssus gallinae (Degeer 1778) \u201d (2018) Universidade Federal de Uberl\u00e2ndia.","DOI":"10.14393\/BJ-v34n1a2018-39599"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213\u2010010\u20100092\u20106"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-50532007000500018"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1590\/1519-6984.286419"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0303878"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140612780"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517\u201083822004000300001"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare10101820"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.04.015"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2023025"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.7213\/cienciaanimal.v5i2.9744"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2014.02.014"},{"key":"e_1_2_10_52_1","volume-title":"Identification of Essential Oil Components by Gas Chromatography\/Mass Spectrometry","author":"Adams R. P.","year":"2017"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm9509410"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.04.013"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(98)00130-8"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1159\/000151267"},{"key":"e_1_2_10_57_1","first-page":"388","volume-title":"Micologia m\u00e9dica \u00e0 luz de autores contempor\u00e2neos","author":"Sidrim J. J. C.","year":"2010"},{"key":"e_1_2_10_58_1","unstructured":"J. M.Mendes \u201cInvestiga\u00e7\u00e3o da atividade antif\u00fangica do \u00f3leo essencial deEugenia caryophyllata Thunb.sobre cepas deCandida tropicalis \u201d 78p. Tese de Doutorado. Disserta\u00e7\u00e3o (Mestrado em Produtos Naturais e Sint\u00e9ticos Bioativos\u2010Concentra\u00e7\u00f5es: Farmacologia) (Universidade Federal da Para\u00edba 2011)."}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71374","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71374","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T19:20:10Z","timestamp":1783365610000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,30]]},"references-count":57,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/cbdv.71374"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71374","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,30]]},"assertion":[{"value":"2025-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71374"}}