{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:14:09Z","timestamp":1783368849660,"version":"3.54.6"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study investigated the antioxidant properties and enzyme\u2010inhibitory activities of\n                    <jats:italic>Cousinia cirsioides<\/jats:italic>\n                    extracts and sub\u2010extracts, along with their phytochemical composition. In this study, the aerial parts of\n                    <jats:italic>C. cirsioides<\/jats:italic>\n                    were extracted with 70% methanol and fractionated into n\u2010hexane, ethyl acetate (CSE), n\u2010butanol, and water sub\u2010extracts. Enzyme inhibition activities were tested against \u03b1\u2010amylase, \u03b1\u2010glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase. Antioxidant capacities were evaluated using DPPH, ABTS, FRAP, and CUPRAC assays. TPC, TFC and LC\u2013HRMS analyses were used to identify the phytochemical profile of the extracts. Additionally, the antimicrobial activity was investigated using the microdilution method. As a result, the CSE sub\u2010extract exhibited the most potent antioxidant and enzyme inhibitory activities among the extracts. It showed significant inhibition of \u03b1\u2010amylase (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0167.967\u00a0\u00b5g\/mL), AChE (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0134.165\u00a0\u00b5g\/mL), and tyrosinase (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0122.354\u00a0\u00b5g\/mL), among others. Moreover, a moderate antimicrobial effect was found in n\u2010hexane sub\u2010extract. LC\u2013HRMS analysis revealed high levels of isoquercitrin, caffeic acid, chlorogenic acid, and protocatechuic acid in the active sub\u2010extract. These compounds were also subjected to the same bioactivity tests. It is revealed that these compounds demonstrated individual bioactivities, although less potent than the extract, suggesting synergistic effects.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71398","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:07:04Z","timestamp":1781708824000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting Metabolic and Neurodegenerative Enzymes With\n                    <i>Cousinia cirsioides<\/i>\n                    Boiss. and Bal. Extracts and Major Compounds: LC\u2013HRMS\u2010Based Phytochemistry and Bioactivity Assessment"],"prefix":"10.1002","volume":"23","author":[{"given":"Hatice \u015eevval","family":"Ceyhan","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3860-7222","authenticated-orcid":false,"given":"Leyla","family":"Pa\u015fayeva","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7123-8396","authenticated-orcid":false,"given":"Hanifa","family":"Fatullayev","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ufuk","family":"\u0130nce","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-7648","authenticated-orcid":false,"given":"Osman","family":"Tugay","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany Faculty of Pharmacy Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u20102011\u2010101392"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13010159"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.31274"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10406638.2023.2264449"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10406638.2023.2233666"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-823974-2.00003-6"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.3c00005"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.2c00237"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-92196-5_4"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.30848\/PJB2020\u20101(43)"},{"key":"e_1_2_11_12_1","first-page":"149","article-title":"T\u00fcrkiye bitkileri listesi:(damarl\u0131 bitkiler)","author":"G\u00fcner A.","year":"2012","journal-title":"Nezahat G\u00f6kyi\u01e7it Botanik Bah\u00e7esi Yay\u0131nlar\u0131"},{"key":"e_1_2_11_13_1","first-page":"15","article-title":"Cytotoxic Effect of Cousinia verbascifolia Bunge Against OVCAR\u20103 and HT\u201029 Cancer Cells","volume":"4","author":"Sajjadi S. 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