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Secondary metabolites from cold\u2010adapted microorganisms may provide unique antimicrobial compounds. Ethyl\u2010acetate extract of\n                    <jats:italic>Alcaligenes pakistanensis<\/jats:italic>\n                    LTP10 from Passu glacier was explored for antimicrobial potential against clinical isolates. Cytotoxicity was determined by Brine shrimp lethality assay. The extract is analyzed by LC\u2010MS\/MS and the data is processed by MZmine. Important compounds were evaluated by molecular docking and in silico study. The extract demonstrated activity against clinical isolates\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    with zone of inhibition ranging from 16\u00a0mm to 24\u00a0mm, with no killing of nauplii suggested non\u2010cytotoxic nature. LC\u2010MS\/MS analysis established presence of important putative antimicrobial metabolites (\n                    <jats:italic>E<\/jats:italic>\n                    )\u20103\u2010(acetyloxymethyl)\u20105\u2010(2\u2010formyl\u20104\u2010hydroxy\u20105,5,8a\u2010trimethyl\u20101,4,4a,6,7,8\u2010hexahydronaphthalen\u20101\u2010yl)pent\u20102\u2010enoic acid, cyclizidine\u2010F, neovasipyridone\u2010G, paenibacillin\u2010A, and tricholomenyn\u2010A. Molecular docking study of these metabolites by AutoDock Vina against dihydrofolate reductase of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    demonstrated binding affinities from \u22126.8\u00a0kcal\/mol to \u22128.5\u00a0kcal\/mol, while against enoyl\u2010ACP reductase of\n                    <jats:italic>E. coli<\/jats:italic>\n                    showed binding affinity values from \u22126.3\u00a0kcal\/mol to \u22127.9\u00a0kcal\/mol. In silico analysis predicted considerable absorption, distribution, metabolism, excretion, safety, and druglikeness properties. These results suggest that metabolites from\n                    <jats:italic>A. pakistanensis<\/jats:italic>\n                    LTP10 possess antimicrobial potential and warrant advanced post\u2010docking validation via molecular dynamics, free\u2010energy, and mechanistic analyses for future antibiotic development.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71402","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T15:47:06Z","timestamp":1781884026000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Glacier to In Silico: Integrated LC\u2010MS Metabolomics and Multi\u2010Level Computational Prioritization of Antimicrobial Metabolites From\n                    <i>Alcaligenes pakistanensis<\/i>\n                    LTP10"],"prefix":"10.1002","volume":"23","author":[{"given":"Imran","family":"Rabbani","sequence":"first","affiliation":[{"name":"Department of Pharmacy Kohat University of Science and Technology  Kohat Pakistan"},{"name":"Department of Microbiology Quaid i Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6098-4142","authenticated-orcid":false,"given":"Muhammad","family":"Rafiq","sequence":"additional","affiliation":[{"name":"Balochistan University of IT, Engineering and Management Sciences  Quetta Pakistan"},{"name":"Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control &amp; Key Laboratory of Environmental Pollution Monitoring and Disease Control Ministry of Education of Guizhou &amp; School of Basic Medical Science &amp; Institution of One Health Research Guizhou Medical University  Gui'an P. 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