{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:14:46Z","timestamp":1783368886725,"version":"3.54.6"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007335","name":"Ministry of Higher Education and Scientific Research","doi-asserted-by":"publisher","award":["D01N01UN230120220003"],"award-info":[{"award-number":["D01N01UN230120220003"]}],"id":[{"id":"10.13039\/501100007335","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000240","name":"New Brunswick Innovation Foundation","doi-asserted-by":"publisher","award":["RPI_2024_020"],"award-info":[{"award-number":["RPI_2024_020"]}],"id":[{"id":"10.13039\/501100000240","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102022\u201003950"],"award-info":[{"award-number":["RGPIN\u20102022\u201003950"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Royal jelly (RJ) is a chemically complex bee\u2010derived secretion whose composition and biological properties are influenced by honeybee genetics, geography, and processing. Native Algerian honeybee subspecies of the African lineage (\n                    <jats:italic>Apis mellifera intermissa<\/jats:italic>\n                    and\n                    <jats:italic>Apis mellifera sahariensis<\/jats:italic>\n                    ) represent an underexplored source of RJ with potential chemical distinctiveness. In this study, fresh and lyophilized RJ samples from three geographically contrasting Algerian regions were characterized using selected chemical markers to assess biodiversity\u2010 and processing\u2010related effects. Chemical analyses revealed marked regional variability in 10\u2011hydroxy\u20112\u2011decenoic acid, with fresh samples containing 1.9%\u20133.0% (w\/w) and lyophilized samples reaching 4.9%\u20138.4% (w\/w). Estimated total phenolic and flavonoid contents also varied by region. Fresh and lyophilized samples showed comparable patterns for the measured markers, supporting the use of both forms for comparative studies. Biological evaluation revealed weak antiradical and antioxidant activities. In cell\u2010based assays, lyophilized RJ selectively reduced Reh cell viability, while Jurkat cells were largely unaffected, with effects attributable to nonspecific cytotoxicity. RJ showed little to no inhibition of leukotriene biosynthesis in a whole\u2010cell 5\u2011lipoxygenase assay, indicating limited anti\u2010inflammatory activity via this pathway. By integrating selected chemical characterization with biologically relevant cellular assays, this work provides a balanced and mechanistically informed assessment of RJ bioactivity.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71406","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T18:40:11Z","timestamp":1781203211000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Markers and Bioactivity of Algerian Royal Jelly: Influence of Bee Subspecies, Geography, and Lyophilization"],"prefix":"10.1002","volume":"23","author":[{"given":"A. S.","family":"Ayad","sequence":"first","affiliation":[{"name":"Pharmaceutical Sciences Research Center (CRSP)  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. P. A.","family":"H\u00e9bert","sequence":"additional","affiliation":[{"name":"New Brunswick Centre For Precision Medicine  Moncton New Brunswick Canada"},{"name":"Department of Chemistry and Biochemistry Universit\u00e9 De Moncton  Moncton New Brunswick Canada"},{"name":"New Brunswick Institute of Health Research Moncton New Brunswick Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Doiron","sequence":"additional","affiliation":[{"name":"New Brunswick Centre For Precision Medicine  Moncton New Brunswick Canada"},{"name":"Department of Chemistry and Biochemistry Universit\u00e9 De Moncton  Moncton New Brunswick Canada"},{"name":"New Brunswick Institute of Health Research Moncton New Brunswick Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Loucif\u2010Ayad","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Biology, Faculty of Sciences Badji Mokhtar University  Annaba Algeria"},{"name":"Faculty of Medicine Badji Mokhtar University  Annaba Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Daas","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Biology, Faculty of Sciences Badji Mokhtar University  Annaba Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-3313","authenticated-orcid":false,"given":"G.","family":"Smagghe","sequence":"additional","affiliation":[{"name":"Institute of Entomology Guizhou University  Guiyang China"},{"name":"Department of Biology Vrije Universiteit Brussel (VUB)  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3744-8012","authenticated-orcid":false,"given":"M.","family":"Touaibia","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Universit\u00e9 De Moncton  Moncton New Brunswick Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4662-5850","authenticated-orcid":false,"given":"M. E.","family":"Surette","sequence":"additional","affiliation":[{"name":"New Brunswick Centre For Precision Medicine  Moncton New Brunswick Canada"},{"name":"Department of Chemistry and Biochemistry Universit\u00e9 De Moncton  Moncton New Brunswick Canada"},{"name":"New Brunswick Institute of Health Research Moncton New Brunswick Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"91","article-title":"Quality Parameters and Nutritional Value of Different Commercial Bee Products","volume":"67","author":"Bobis O. 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