{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:15:03Z","timestamp":1783368903310,"version":"3.54.6"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007684","name":"Ministry of Education and Science of Ukraine","doi-asserted-by":"publisher","award":["0126U001562"],"award-info":[{"award-number":["0126U001562"]}],"id":[{"id":"10.13039\/501100007684","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A strategy has been developed for identification of biologically active compounds among unknown benzo[\n                    <jats:italic>e<\/jats:italic>\n                    ][1,2,4]triazolo[1,5\u2010\n                    <jats:italic>c<\/jats:italic>\n                    ][1,2,3]triazines, based on the application of conformational restriction in the series of ([2\u2010(3\u2010R\u20101\n                    <jats:italic>H<\/jats:italic>\n                    \u2010[1,2,4]triazol\u20105\u2010yl)phenyl]amines with known chemotherapeutic activity. The target compounds were synthesized by diazotization of the corresponding ([2\u2010(3\u2010R\u20101\n                    <jats:italic>H<\/jats:italic>\n                    \u2010[1,2,4]triazol\u20105\u2010yl)phenyl]amines followed by cyclization of the formed diazo\u2010compounds. The structures of products have been proven by IR, LC\u2013MS, NMR spectroscopy, and x\u2010ray structural analysis. Cytotoxicity screening was performed on a panel of cell lines derived from various tumor types and pseudo\u2010normal cell lines. It was demonstrated that benzo[\n                    <jats:italic>e<\/jats:italic>\n                    ][1,2,4]triazolo[1,5\u2010\n                    <jats:italic>c<\/jats:italic>\n                    ][1,2,3]triazines have selective antiproliferative activity against Jurkat cell lines with IC\n                    <jats:sub>50<\/jats:sub>\n                    ranging from 7.64 to &gt;100\u00a0\u00b5M. We identified 2\u2010(naphthaline\u20101\u2010ylmethyl)benzo[\n                    <jats:italic>e<\/jats:italic>\n                    ][1,2,4]triazolo[1,5\u2010\n                    <jats:italic>c<\/jats:italic>\n                    ][1,2,3]triazine (\n                    <jats:bold>2e<\/jats:bold>\n                    ) with high cytotoxic activity against this cell line with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 7.64\u00a0\u00b5M. It is noteworthy that the conformational restrictions imposed proved to be a promising strategy for searching for biologically active compounds, and additional structural modification may lead to more effective and promising antitumor agents.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71416","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:44:02Z","timestamp":1781847842000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Benzo[\n                    <i>e<\/i>\n                    ][1,2,4]triazolo[1,5\u2010\n                    <i>c<\/i>\n                    ][1,2,3]triazines: Design, Synthesis, and Cytotoxic Effects"],"prefix":"10.1002","volume":"23","author":[{"given":"Serhii","family":"Sinyavskyi","sequence":"first","affiliation":[{"name":"Institute of Chemistry and Geology Oles Honchar Dnipro National University  Dnipro Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svitlana","family":"Koptieva","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Geology Oles Honchar Dnipro National University  Dnipro Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleksii","family":"Voskoboinik","sequence":"additional","affiliation":[{"name":"National University \u00abZaporizhzhia Polytechnic\u00bb  Zaporizhzhia Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8048-445X","authenticated-orcid":false,"given":"Oleksii","family":"Antypenko","sequence":"additional","affiliation":[{"name":"Zaporizhzhia State Medical and Pharmaceutical University  Zaporizhzhia Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Bohdan","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliia","family":"Kozak","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology of the National Academy of Sciences of Ukraine  Lviv Ukraine"},{"name":"Molecular Design Center Danylo Halytsky Lviv National Medical University  Lviv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Shishkina","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serhii","family":"Kovalenko","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Geology Oles Honchar Dnipro National University  Dnipro Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570193X18666210122154419"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41061-022-00385-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12450"},{"issue":"4","key":"e_1_2_10_5_1","first-page":"491","article-title":"Review on Triazines Energetic Compounds","volume":"20","author":"Zhang X. 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