{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:05:17Z","timestamp":1785330317460,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Phytochemical investigation on the stems of\n                    <jats:italic>Nerium oleander<\/jats:italic>\n                    led to the isolation of four previously undescribed polyene pregnane steroids, namely neristerone A\u2010D (\n                    <jats:bold>1\u20134<\/jats:bold>\n                    ), together with six known analogues (\n                    <jats:bold>5\u201310<\/jats:bold>\n                    ). Their structures were fully elucidated by extensive spectroscopic analysis (UV, IR, HR\u2010ESI\u2010MS, and NMR) and electronic circular dichroism (ECD) calculations. All isolates were evaluated for the inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)\u2010induced RAW264.7 macrophages. Notably, compounds\n                    <jats:bold>1<\/jats:bold>\n                    ,\n                    <jats:bold>4<\/jats:bold>\n                    , and\n                    <jats:bold>10<\/jats:bold>\n                    exhibited significant inhibitory activity with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 11.27 \u00b1 0.81 to 18.13 \u00b1 1.25\n                    <jats:italic>\u00b5<\/jats:italic>\n                    mol\/L, which were lower than that of the positive control dexamethasone (21.48 \u00b1 0.83\n                    <jats:italic>\u00b5<\/jats:italic>\n                    mol\/L). A preliminary structure\u2013activity relationship analysis was also discussed. This study expands the chemical diversity of polyene pregnane steroids from\n                    <jats:italic>N. oleander<\/jats:italic>\n                    and provides new insights into their anti\u2011inflammatory potency.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71446","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:54:08Z","timestamp":1784130848000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["New Polyene Pregnane Steroids From the Stems of\n                    <i>Nerium oleander<\/i>\n                    : Structural Characterization and In Vitro Anti\u2010Inflammatory Evaluation"],"prefix":"10.1002","volume":"23","author":[{"given":"Tong\u2010Xin","family":"Zheng","sequence":"first","affiliation":[{"name":"Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources School of Ocean and Tropical Medicine Guangdong Medical University  Zhanjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Xin","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources School of Ocean and Tropical Medicine Guangdong Medical University  Zhanjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u2010Hui","family":"Zhan","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources School of Ocean and Tropical Medicine Guangdong Medical University  Zhanjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Hong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Medical University  Dongguan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing\u2010Wei","family":"Li","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine Guangdong Medical University  Zhanjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"The First School of Clinical Medicine Guangdong Medical University  Zhanjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Medical University  Dongguan P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2926-4635","authenticated-orcid":false,"given":"Yong\u2010Mei","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources School of Ocean and Tropical Medicine Guangdong Medical University  Zhanjiang P.R. China"},{"name":"The Marine Biomedical Research Institute of Guangdong Zhanjiang  Zhanjiang Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8617-5968","authenticated-orcid":false,"given":"Xiao\u2010San","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources School of Ocean and Tropical Medicine Guangdong Medical University  Zhanjiang P.R. China"},{"name":"The Marine Biomedical Research Institute of Guangdong Zhanjiang  Zhanjiang Guangdong P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6121-1715","authenticated-orcid":false,"given":"Hui","family":"Luo","sequence":"additional","affiliation":[{"name":"Guangdong Engineering Technology Research Center for the Development and Utilization of Mangrove Wetland Medicinal Resources School of Ocean and Tropical Medicine Guangdong Medical University  Zhanjiang P.R. China"},{"name":"The Marine Biomedical Research Institute of Guangdong Zhanjiang  Zhanjiang Guangdong P.R. 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