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Burkill, investigate its antitumor activity and molecular mechanism against MDA\u2010MB\u2010231 and BT\u2010549 breast cancer cell lines. The glycoprotein DeGP\u20104 was isolated, which contained 48.35% carbohydrates and 50.20% proteins. DeGP\u20104 was identified as glycoprotein around 23.28 kDa, a purity of 93.20%, and contained 16 different amino acids, in which the Asp and Glu were predominant. The carbohydrate chain composition was predominantly mannose (53.84%), glucose (43.38%), galactose (2.08%), and arabinose (0.99%), and two O\u2010glycosylation sites were identified at positions 94 and 146. DeGP\u20104 demonstrated potent inhibitory effects on the proliferation of MDA\u2010MB\u2010231 and BT\u2010549 breast cancer cells, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 1.2 and 2.5 \u00b5g\/mL. The inhibitory effect of DeGP\u20104 on MDA\u2010MB\u2010231 is better than cisplatin, and the inhibitory effect on BT\u2010549 is comparable to cisplatin. DeGP\u20104 may suppress tumor cell migration and induced apoptosis by promoting reactive oxygen species (ROS) production and modulating the expression of key apoptotic proteins, including downregulation of Bcl\u20102 and upregulation of Bax and Caspase\u20103. Furthermore, in vivo experiments conducted the mouse tumor model, supported by histological and immunohistochemical analyses, confirmed the significant antitumor activity of DeGP\u20104.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71450","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T09:13:25Z","timestamp":1782292405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["O\u2010Glycoprotein DeGP\u20104 From\n                    <i>Dioscorea esculenta<\/i>\n                    (Lour.) Burkill: Isolation, Structural Characterization, and Potent Anti\u2010Triple\u2010Negative Breast Cancer Activity"],"prefix":"10.1002","volume":"23","author":[{"given":"Guo","family":"Xu","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education Key Laboratory of Water Pollution Treatment &amp; Resource Reuse of Hainan Province Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education Key Laboratory of Water Pollution Treatment &amp; Resource Reuse of Hainan Province Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education Key Laboratory of Water Pollution Treatment &amp; 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Hainan Provincial Key Laboratory for Tropical Cardiovascular Diseases Research Research Institute for Science and Technology Development Hainan Medical University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oncology Ji'an Central People's Hospital  Ji'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1779-8736","authenticated-orcid":false,"given":"Cai\u2010Juan","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education Key Laboratory of Water Pollution Treatment &amp; Resource Reuse of Hainan Province Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Peng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education Key Laboratory of Water Pollution Treatment &amp; 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