{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:05:30Z","timestamp":1785330330941,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004909","name":"Universidade Federal do Rio Grande do Sul","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004909","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Dysphania ambrosioides<\/jats:italic>\n                    , a prominent medicinal plant, exhibits diverse biological properties with emerging potential in cancer research. Despite its therapeutic promise, the relationship between phytochemical composition and biological activities remains insufficiently explored, particularly regarding geographical variation. This study investigates the correlation between the phytochemical profiles of\n                    <jats:italic>D. ambrosioides<\/jats:italic>\n                    extracts from Colombia (DA\u2010Col) and Brazil (DA\u2010Bra) and their cytotoxic activity against HeLa, SiHa, and MCF\u20107 cancer cell lines. Qualitative chemical profiling revealed distinct presence\u2010and\u2010absence patterns of specific secondary metabolites between the two geographical sources. These geographically driven variations in the phytochemical profile directly influenced the observed biological activity, with the extracts exhibiting differential cytotoxicity profiles. Specifically, Colombian extract DA\u2010Col\u20101 showed significant activity against HeLa cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a03.09\u00a0\u00a0\u00b1\u00a00.32 \u00b5g\/mL) and DA\u2010Col\u20102 demonstrated potent effects on MCF\u20107 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a02.08\u00a0\u00a0\u00b1\u00a00.58 \u00b5g\/mL). Brazilian extract DA\u2010Bra\u20102 selectively inhibited MCF\u20107 proliferation (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a03.09\u00a0\u00a0\u00b1\u00a00.12 \u00b5g\/mL). Apoptosis assays indicated that these extracts might induce early apoptotic events, suggesting a potential mechanism of action that warrants further investigation. This study establishes a direct link between the chemical composition of geographically distinct\n                    <jats:italic>D. ambrosioides<\/jats:italic>\n                    samples and their selective anticancer potential, providing a foundation for natural product\u2010based drug development targeting specific cancer types.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71452","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:39:11Z","timestamp":1783611551000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of the Cytotoxic Effect of\n                    <i>Dysphania ambrosioides<\/i>\n                    Extracts Against Human Breast and Cervical Cancer Cells"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1839-9685","authenticated-orcid":false,"given":"Andres Camilo Moreno","family":"Abadia","sequence":"first","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis (LaFiS) Federal University of Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2781-2490","authenticated-orcid":false,"given":"Lina Marcela","family":"Trujillo Chac\u00f3n","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Sustancias Bioactivas Facultad de ciencias farmac\u00e9uticas y alimentarias Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Biz","family":"Willig","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical and Cytological Analyses (LABC) School of Pharmacy Federal University of Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9821-3420","authenticated-orcid":false,"given":"Edison","family":"Osorio","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Sustancias Bioactivas Facultad de ciencias farmac\u00e9uticas y alimentarias Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6773-6480","authenticated-orcid":false,"given":"Diogo Andr\u00e9","family":"Pilger","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical and Cytological Analyses (LABC) School of Pharmacy Federal University of Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1288-2293","authenticated-orcid":false,"given":"Miriam Anders","family":"Apel","sequence":"additional","affiliation":[{"name":"Pharmacognosy Laboratory Federal University of Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8806-3182","authenticated-orcid":false,"given":"Simone Cristina Baggio","family":"Gnoatto","sequence":"additional","affiliation":[{"name":"Laboratory of Phytochemistry and Organic Synthesis (LaFiS) Federal University of Rio Grande do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.17129\/botsci.2715"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-13401-7_14"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27134060"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21050559"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010019\u201009629\u20109"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.11648\/j.jps.s.2015030101.18"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010021\u201009752\u20106"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800018-2.00022-4"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2022.106393"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20103\u2010319\u201013401\u20107_14"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-28933-1_106"},{"issue":"2","key":"e_1_2_11_13_1","first-page":"267","article-title":"\u00d3leo Essencial de Chenopodium ambrosioides L.: Estado Da Arte","volume":"36","author":"S\u00e1 R. 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