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More effective, less toxic, and affordable compounds are needed. Thus, discovering such compounds could significantly enhance the management and control of cutaneous leishmaniasis. Brazil has a biodiversity of flora and microorganisms that remains unexplored, mainly for evaluating extracts and compounds with anti\u2010\n                    <jats:italic>Leishmania<\/jats:italic>\n                    activity. This study aimed to screen the leishmanicidal activity of extracts and fractions from leaves of\n                    <jats:italic>Eugenia pyriformis<\/jats:italic>\n                    Cambess. collected during different seasons (winter and summer) in Rio de Janeiro, Brazil. Extracts from summer\u2010collected leaves showed higher effectiveness against\n                    <jats:italic>Leishmania (Leishmania) amazonensis<\/jats:italic>\n                    promastigotes and amastigotes. The IC\n                    <jats:sub>50<\/jats:sub>\n                    values for the extract from leaves collected in summer (Ep1) were significantly lower than those for winter extracts. Extracts showed low toxicity to human macrophages and a high selectivity index (SI &gt; 12). In addition, the Ep1 extract induced G0\/G1 phase arrest and necrosis in promastigotes. UHPLC\u2010HR\u2010ESI\u2010MS\/MS analysis combined with molecular networking revealed that flavonoids and terpenoids were predominant in active fractions. The study highlights the potential of\n                    <jats:italic>E. pyriformis<\/jats:italic>\n                    Cambess. as a source of leishmanicidal compounds from leaves collected during the summer season.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71471","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:19:39Z","timestamp":1783369179000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leishmanicidal Potential of\n                    <i>Eugenia pyriformis<\/i>\n                    Cambess. (Myrtaceae) Leaf Extracts: Metabolomics and Mechanism of Action"],"prefix":"10.1002","volume":"23","author":[{"given":"Rog\u00e9ria C.","family":"Zauli","sequence":"first","affiliation":[{"name":"Postgraduate Program in Chemical Biology Federal University of S\u00e3o Paulo \u2010 UNIFESP  S\u00e3o Paulo Brazil"},{"name":"Institute of Environmental Chemical and Pharmaceutical Sciences Department of Pharmaceutical Sciences Laboratory of Cellular Immunology and Biochemistry of Fungi and Protozoa Federal University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Clara Lima Nunes","family":"Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Chemical Biology Federal University of S\u00e3o Paulo \u2010 UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Sartorelli","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Chemical Biology Federal University of S\u00e3o Paulo \u2010 UNIFESP  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Gon\u00e7ales","family":"Rando","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Chemical Biology Federal University of S\u00e3o Paulo \u2010 UNIFESP  S\u00e3o Paulo Brazil"},{"name":"Institute of Environmental Chemical and Pharmaceutical Sciences Department of Pharmaceutical Sciences Laboratory of Cellular Immunology and Biochemistry of Fungi and Protozoa Federal University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aislan Cristina Rheder Fagundes","family":"Pascoal","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Nova Friburgo Health Institute Federal Fluminense University \u2010 UFF  Rio de Janeiro 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