{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:05:12Z","timestamp":1785330312486,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Crassulacean Acid Metabolism (CAM) plants possess unique stress\u2010adaptive physiologies, yet their chemotype\u2010bioactivity relationships remain poorly understood. We established an integrated workflow combining GC\u2013MS metabolomics, 2D\/3D functional validation, and in silico profiling to evaluate hydrothermal extracts from four CAM species (BDAE [\n                    <jats:italic>Bulbophyllum drymoglossum<\/jats:italic>\n                    ], CRSAE [\n                    <jats:italic>Crassula rupestris<\/jats:italic>\n                    ], ECAE [\n                    <jats:italic>Echeveria<\/jats:italic>\n                    ], and STAE [\n                    <jats:italic>Hylotelephium telephium<\/jats:italic>\n                    ]). PCA and marker analysis resolved three distinct chemotypes: acid\u2010rich, sugar\u2010rich, and lipid\/sterol\u2010enriched. Using standardized noncytotoxic concentrations, we demonstrated that acid\u2010rich extracts (BDAE\/CRSAE) most consistently attenuated H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced oxidative stress in NIH\/3T3 fibroblasts. These cytoprotective effects included significant ROS reduction and preservation of mitochondrial membrane potential. Importantly, these antioxidant properties were robustly retained in a 3D bioprinted tissue\u2010mimetic model with high biocompatibility, as confirmed by live\/dead imaging. Furthermore, in silico docking justifies prioritizing phaselic acid as a bioactive marker, showing reproducible interactions with Keap1, COX\u20102, and MMP\u20101. While acknowledging the specific analytical scope of GC\u2013MS and the predictive nature of computational modeling, this study successfully connects chemical composition, biological activity, and mechanistic hypotheses within a robust chemotype\u2010resolved framework. Our findings offer a strategic scientific rationale for the selective application of CAM\u2010derived metabolites in antioxidant strategies.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71484","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T07:52:00Z","timestamp":1783497120000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemotype\u2010Resolved Cytoprotection of CAM Hydrothermal Extracts Validated in 2D\/3D Fibroblast Models and Supported by In Silico Target Profiling"],"prefix":"10.1002","volume":"23","author":[{"given":"Eun Hye","family":"Park","sequence":"first","affiliation":[{"name":"Department of Biotechnology College of Life and Health Sciences Hoseo University  Asan Chungnam Republic of Korea"},{"name":"CANDIGEN Co., Ltd. Beonyeong\u2010ro  Cheonan\u2010si Chungcheongnam\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4590-3644","authenticated-orcid":false,"given":"Sung\u2010Jo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology College of Life and Health Sciences Hoseo University  Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.70268"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.11.073"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcad160"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1615338"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2015.04.001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac303"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9912436"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac303"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.70117"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01197-1"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12953-025-00241-8"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44385-026-00073-x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb3004s114"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.14794"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2018.05.014"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-026-45553-7"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.07.009"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202201505"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2025.110554"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-025-04518-y"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-009-0168-0"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.112967"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.19.0251"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-78357-8"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-025-01997-1"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2024.150561"},{"key":"e_1_2_10_28_1","first-page":"250630","article-title":"Bulbophyllum Drymoglossum Aqueous Extract Modulates Immunometabolism and Oxidative Stress in Porcine Alveolar Macrophages","volume":"39","author":"Park E. 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