{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:04:42Z","timestamp":1785330282291,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study investigates the phytochemical composition, antioxidant potential, and preliminary in silico interactions of the methanolic root extract of\u00a0\n                    <jats:italic>Antigonon leptopus<\/jats:italic>\n                    . Qualitative phytochemical screening suggested the presence of alkaloids, flavonoids, phenolics, tannins, saponins, and terpenoids. The extract exhibited moderate free radical scavenging activity in the DPPH assay, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 142.45\u00a0\u00b1\u00a03.05\u00a0\u00b5g\/mL, compared with ascorbic acid (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a018.09\u00a0\u00b1\u00a00.51\u00a0\u00b5g\/mL). Statistical analysis using Student's t\u2010test indicated a significant difference between the extract and standard (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). In addition, the extract exhibited a ferric reducing antioxidant power (FRAP) of 278.12\u00a0\u00b1\u00a00.65\u00a0\u00b5M Fe\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    \/g dry extract and contained appreciable levels of total phenolics (394.50\u00a0\u00b1\u00a03.77\u00a0mg GAE\/g dry extract) and total flavonoids (55.33\u00a0\u00b1\u00a01.16\u00a0mg QE\/g dry extract), suggesting that polyphenolic constituents contribute to the observed antioxidant activity. Molecular docking was conducted as an exploratory approach to predict potential interactions of selected phytochemicals with NADPH oxidase 2 (NOX2), but these results are considered preliminary and require further experimental validation. Overall, the findings suggest that\u00a0\n                    <jats:italic>A. leptopus<\/jats:italic>\n                    \u00a0roots contain bioactive secondary metabolites with moderate antioxidant activity, warranting further in\u2010depth biological and mechanistic studies.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71511","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T17:42:43Z","timestamp":1785087763000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LC\u2013HRMS Identification and NOX2\u2010Targeted Molecular Docking of Antioxidant Metabolites From\n                    <i>Antigonon leptopus<\/i>\n                    Root Extract"],"prefix":"10.1002","volume":"23","author":[{"given":"Almas","family":"Khanam","sequence":"first","affiliation":[{"name":"Department of Botany M.L.K. P.G. College  Balrampur Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rishi Ranjan","family":"Pandey","sequence":"additional","affiliation":[{"name":"Department of Chemistry M.L.K. P.G. College  Balrampur Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sant Kavi Baba Baijnath Government PG College Harakh  Barabanki Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7353-1025","authenticated-orcid":false,"given":"Mohd.","family":"Akmal","sequence":"additional","affiliation":[{"name":"Department of Botany M.L.K. P.G. College  Balrampur Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines5030093"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8416763"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2024.10.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2006.02.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2025.101651"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/app151810191"},{"key":"e_1_2_8_8_1","first-page":"754","article-title":"A Comprehensive Review on Antigonon Leptopus\u2014A Potential Medicinal Herb","volume":"5","author":"Bhalerao N.","year":"2023","journal-title":"International Research Journal of Modernization in Engineering Technology and Science"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.5530\/ijper.54.2s.82"},{"key":"e_1_2_8_10_1","first-page":"2268","article-title":"Pharmacological Evaluation of Antigonon Leptopus Hook & Arn.: A Promising Plant for Future Therapeutics","volume":"13","author":"Mote A. N.","year":"2025","journal-title":"World Journal of Pharmaceutical Sciences"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.sciaf.2023.e01585","article-title":"A Review of Modern and Conventional Extraction Techniques and Their Applications for Extracting Phytochemicals From Plants","volume":"19","author":"Chibuye B.","year":"2023","journal-title":"Scientific African"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2019.e00370"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43094-020-00161-8"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300750"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/s2175\u201097902019000417129"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15107313"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2021.03.011"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4172\/2573\u20104555.1000193"},{"issue":"2","key":"e_1_2_8_19_1","first-page":"130","article-title":"Flavonoids and Phenolic Acids as Antioxidants in Plants and Human Health","volume":"16","author":"Saxena M.","year":"2012","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"e_1_2_8_20_1","article-title":"Health\u2010Beneficial Phenolic Aldehyde in Antigonon Leptopus Tea\u2032, Evid.\u2010Based Complement","volume":"2011","author":"Mulabagal V.","year":"2011","journal-title":"Alternative Medicine"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12081606"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8070237"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2025.111489"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.11.009"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf070473q"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.14233\/ajchem.2019.22056"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2013.05.014"},{"key":"e_1_2_8_28_1","first-page":"103","article-title":"Medicinal Uses, Phytochemistry and Pharmacological Activities of Antigonon leptopus Hook. and Arn. (Polygonaceae): A Review","volume":"10","author":"Raghavendra H.","year":"2018","journal-title":"Journal of Chemical and Pharmaceutical Research"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.06.013"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.13040\/IJPSR.0975-8232.IJP.5(2).91\u201096"},{"key":"e_1_2_8_31_1","first-page":"10","article-title":"New Steroidal Saponin From Antigonon leptopus Hook. And Arn","volume":"501","author":"Apaya M. K. L.","year":"2014","journal-title":"Pharmacognosy Magazine"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u201014\u201047"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26195956"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants6040042"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.4236\/cm.2013.44018"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.51152\/jbarbiomed.v5i1.29"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891\u20105849(95)02227\u20109"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/np9904509"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.04.038"},{"key":"e_1_2_8_41_1","first-page":"1","article-title":"Comparative Evaluation of Antioxidant Potential of Ornamental Plants Including Antigonon Leptopus","volume":"14","author":"Ramesh B. S.","year":"2020","journal-title":"International Journal of Green Pharmacy"},{"key":"e_1_2_8_42_1","first-page":"134","article-title":"Antioxidant Activity of Polygonum Aviculare L. extract","volume":"105","author":"Zhang X.","year":"2006","journal-title":"Journal of Ethnopharmacology"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.22270\/jddt.v15i4.7082"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/1811199a0"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202504036"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.2196\/14232"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42250\u2010023\u201000766\u20105"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71511","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71511","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T12:23:48Z","timestamp":1785327828000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/cbdv.71511"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71511","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2025-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71511"}}