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This study aimed to evaluate the phytochemical profile, acute toxicity, and genotoxicity of an aqueous extract from leaves of this plant (AoLE). AoLE contained total phenolic content of 266.1\u00a0mg pyrogallol equivalents\/g dry weight (DW), total tannin content of 32.2\u00a0mg tannic acid equivalents\/g DW, 14.58\u00a0mg\/mL proteins, and lectin activity. HPLC analysis revealed gallic acid and rutin presence. LC\u2010ESI\u2010MS identified 13 flavonoids, 6 phenolic acids, and 4 gallotannin derivatives. For toxicological evaluation, mice received a single oral dose of 2000\u00a0mg\/kg. No mortality, behavioral alterations, or changes in body weight, food\/water intake, hematological parameters, or biochemical markers were observed over 14 days. Organ weights and histology of the liver, kidneys, and spleen remained normal, indicating an LD\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0&gt;\u00a02000\u00a0mg\/kg. Genotoxicity assays showed no increase in micronucleate cells or DNA damage in the comet assay. The presence of polyphenols, tannins, flavonoids, and lectins, together with no evidence of acute toxicity under the tested conditions, supports the development of AoLE as a standardized phytopharmaceutical. Future studies should investigate chronic toxicity and long\u2010term stability to advance preclinical and clinical applications.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71528","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T18:02:05Z","timestamp":1785088925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Characterization and In Vivo Safety Assessment of Aqueous Extract From\n                    <i>Anacardium occidentale<\/i>\n                    L. (Anacardiaceae) Leaves"],"prefix":"10.1002","volume":"23","author":[{"given":"Elinado Francisco de Lima","family":"Bento","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica Centro de Bioci\u00eancias Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caio Everton Nunes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Centro de Bioci\u00eancias Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita Giselly","family":"dos Santos Souza","sequence":"additional","affiliation":[{"name":"Centro Acad\u00eamico de Vit\u00f3ria Universidade Federal de Pernambuco  Vit\u00f3ria de Santo Ant\u00e3o Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camylla Janiele Lucas","family":"Ten\u00f3rio","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Centro de Ci\u00eancias da Sa\u00fade Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natanael Teles Ramos","family":"de Lima","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Universidade Federal da Para\u00edba  Jo\u00e3o Pessoa Para\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiano Aparecido","family":"Chagas","sequence":"additional","affiliation":[{"name":"Centro Acad\u00eamico de Vit\u00f3ria Universidade Federal de Pernambuco  Vit\u00f3ria de Santo Ant\u00e3o Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Maria Barbosa","family":"Filho","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Universidade Federal da Para\u00edba  Jo\u00e3o Pessoa Para\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda Rhayanny Assun\u00e7\u00e3o","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Centro de Ci\u00eancias da Sa\u00fade Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Alberto Lira","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Centro de Ci\u00eancias da Sa\u00fade Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel Paulo","family":"Cibulski","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Universidade Estadual da Para\u00edba  Campina Grande Para\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisson Mac\u00e1rio","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Centro de Bioci\u00eancias Universidade Federal de Pernambuco  Recife Pernambuco Brazil"},{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas Universidade Estadual da Para\u00edba  Campina Grande Para\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Henrique","family":"Napole\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica Centro de Bioci\u00eancias Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.06.031"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.52783\/jns.v14.2210"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.53858\/arocpb05010917"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.5380\/dma.v62i0.82153"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-90-481-8661-7_6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/2210315512666220427113702"},{"key":"e_1_2_9_8_1","first-page":"72","article-title":"Evaluation of Antioxidant Potential of Methanolic Leaf Extract of Anacardium occidentale (Linn) on the Testes of Streptozotocin\u2010Induced Diabetic Wistar Rats\"","volume":"17","author":"Ukwenya V.","year":"2013","journal-title":"European Journal of Anatomy"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.03.003"},{"key":"e_1_2_9_10_1","first-page":"1","article-title":"Hypoglycemic Effect of Anacardium occidentale L. 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