{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:05:48Z","timestamp":1785330348553,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T00:00:00Z","timestamp":1785024000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp; Biodiversity"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to investigate the antifungal activity of the vanillin\u2010derived Schiff base complexed with zinc (II) (SBZ) against fluconazole\u2010resistant strains of\n                    <jats:italic>Candida<\/jats:italic>\n                    spp. and\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    . The broth microdilution assay was performed to determine the minimum inhibitory concentration (MIC). The checkerboard assay was used to evaluate the type of pharmacological interaction of the combination of SBZ with azoles. Flow cytometry and alkaline comet assays were performed to investigate the possible action mechanism of SBZ against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    . The MIC values of SBZ for\n                    <jats:italic>Candida<\/jats:italic>\n                    spp. ranged from 24 to 74.7\u00a0\u00b5g\/mL and for\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    from 32 to 53.3\u00a0\u00b5g\/mL. The combination of SBZ with both fluconazole and itraconazole resulted in additive and indifferent interactions, respectively. The mechanism of action appears to involve mitochondrial depolarization, increased oxidative stress, and DNA damage, leading to apoptosis. Thus, the SBZ complex shows promising in vitro antifungal activity, and its combination with azoles may contribute to optimizing the efficacy of these drugs, justifying future in vivo investigations.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71538","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T17:57:32Z","timestamp":1785088652000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antifungal Activity and Possible Mechanism of Action of the Zn (II) Complex With a Schiff Base Against Fluconazole\u2010Resistant Strains of\n                    <i>Candida<\/i>\n                    spp. and\n                    <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0619-2782","authenticated-orcid":false,"given":"L\u00edvia Gurgel do Amaral Valente","family":"S\u00e1","sequence":"first","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Laboratory For Bioprospecting in Antimicrobial Molecules (LABIMAN), Center For Drug Research and Development (NPDM) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia Rocha da","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Laboratory For Bioprospecting in Antimicrobial Molecules (LABIMAN), Center For Drug Research and Development (NPDM) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Batista de Andrade","family":"Neto","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Laboratory For Bioprospecting in Antimicrobial Molecules (LABIMAN), Center For Drug Research and Development (NPDM) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Christus University (Unichristus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Sampaio","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Laboratory For Bioprospecting in Antimicrobial Molecules (LABIMAN), Center For Drug Research and Development (NPDM) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Christus University (Unichristus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara Elloyse Almeida Moreira","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Laboratory For Bioprospecting in Antimicrobial Molecules (LABIMAN), Center For Drug Research and Development (NPDM) Federal University of Cear\u00e1 (UFC)  Fortaleza Cear\u00e1 Brazil"},{"name":"Christus University (Unichristus)  Fortaleza Cear\u00e1 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