{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:08:58Z","timestamp":1788858538170,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Propolis has long been used in Algerian folk medicine to strengthen immunity and treat infections. This work investigated the immunomodulatory effects of an ethanolic extract of propolis (EEP) on human lymphocytes. Brown propolis samples were collected from the Tlemcen region of Algeria. Human peripheral blood lymphocytes from healthy donors were cultured with increasing concentrations of EEP (0.25\u20132\u00a0mg\/mL). Cell proliferation, cytotoxicity, ATP, cytokine secretion (IL\u20102, IFN\u2010\u03b3, and IL\u20104), and redox markers (MDA, carbonylated proteins, GSH, catalase, and NO) were assessed. EPP exhibited a biphasic response: moderate concentrations enhanced lymphocyte proliferation, cytokine secretion, and antioxidant defenses, while higher concentrations induced cytotoxicity, Th1\u2010dominant response and oxidative stress associated to decreased catalase activity. Molecular modeling revealed that caffeic acid, quercetin, and catechin, the major EPP compounds, exhibit strong binding affinity of toward catalase and interact with critical residues involved in enzymatic activity suggesting their potential involvement in catalase\u2010related redox modulation with favorable pharmacokinetic properties.<\/jats:p>","DOI":"10.1002\/cbdv.71554","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T06:21:03Z","timestamp":1785824463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biphasic Regulation of Human Lymphocyte Redox Status and Cytokine Secretion by Algerian Propolis Extract: In Vitro and In Silico Evaluations"],"prefix":"10.1002","volume":"23","author":[{"given":"Yassamine Ikram","family":"Soltani","sequence":"first","affiliation":[{"name":"Laboratory of Organic Chemistry Natural Products and Analysis COSNA, Department of Chemistry, Faculty 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Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, Physiopathology and Biochemistry of Nutrition University Abou\u2010Bekr Belkaid  Tlemcen Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Chelihi","sequence":"additional","affiliation":[{"name":"Laboratory of Catalysis and Synthesis in Organic Chemistry, Faculty of Sciences University Abou\u2010Bekr Belkaid  Tlemcen Algeria"},{"name":"Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology Silesian University of Technology  Gliwice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed El Amine","family":"Dib","sequence":"additional","affiliation":[{"name":"Laboratory of Organic Chemistry Natural Products and Analysis COSNA, Department of Chemistry, Faculty of Sciences University Abou\u2010Bekr Belkaid  Tlemcen Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoheir","family":"Arrar","sequence":"additional","affiliation":[{"name":"Laboratory of Organic Chemistry Natural Products and Analysis COSNA, Department of Chemistry, Faculty of Sciences University Abou\u2010Bekr Belkaid  Tlemcen Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nassima Amel Soulimane","family":"Mokhtari","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology, Department of Biology, Faculty of Natural and Life Sciences, Earth and Universe, Physiopathology and Biochemistry of Nutrition University Abou\u2010Bekr Belkaid  Tlemcen 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