{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:08:59Z","timestamp":1788858539050,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Melanoma cells exhibit characteristic vulnerabilities in redox homeostasis and metabolic plasticity, making these processes attractive targets for natural\u2010product\u2010based intervention. In the present study, the aqueous extract of\n                    <jats:italic>Echeveria<\/jats:italic>\n                    \u201cBlue Curls\u201d (BCAE) was investigated through integrated phytochemical profiling, computational analysis, and cellular assays in B16\u2010F10 melanoma cells. Untargeted GC\u2013MS analysis tentatively identified eight putative metabolites, predominantly saccharides and organic acids, while molecular docking generated hypothetical binding models between selected constituents and the catalytic domain of NADPH oxidase 2 (NOX2). BCAE treatment was associated with reduced intracellular reactive oxygen species (ROS) and mitochondrial superoxide, accompanied by changes in NOX2 protein abundance and antioxidant\u2010related protein expression. These responses coincided with mitochondrial depolarization, altered expression of glycolysis\u2010, fatty acid oxidation\u2010, and mitochondrial bioenergetic\u2010related genes, reduced intracellular lipid accumulation, increased expression of apoptosis\u2010associated genes, and impaired migratory capacity. In contrast, no significant reduction in cell viability was observed in NIH\/3T3 fibroblasts under the same conditions. Collectively, these findings indicate that BCAE exposure is associated with coordinated alterations in redox status, mitochondrial function, and metabolism\u2010related cellular responses in melanoma cells. Because this study relied primarily on transcriptional profiling, computational prediction, and in vitro analyses, the findings should be regarded as exploratory and hypothesis\u2010generating.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71555","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T03:47:23Z","timestamp":1785556043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Characterization of\n                    <i>Echeveria<\/i>\n                    \u201cBlue Curls\u201d and Its Association With Redox\u2013Metabolic Perturbations in Melanoma Cells"],"prefix":"10.1002","volume":"23","author":[{"given":"Eun Hye","family":"Park","sequence":"first","affiliation":[{"name":"Department of Biotechnology, College of Life and Health Sciences Hoseo University  Asan Chungnam Republic of Korea"},{"name":"CANDIGEN Co., Ltd. Beonyeong\u2010ro  Cheonan\u2010si Chungcheongnam\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4590-3644","authenticated-orcid":false,"given":"Sung\u2010Jo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life and Health Sciences Hoseo University  Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u201000114\u2010z"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwac206"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210\u2010024\u201003622\u20106"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2np00008c"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010024\u201000730\u20102"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010021\u201000822\u20106"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010023\u201028620\u20108"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2024.101966"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010025\u201002311\u2010x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15068"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010026\u201002583\u2010x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010022\u201003421\u2010z"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010023\u201002907\u2010z"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.10.008"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010025\u201002253\u20104"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010025\u201002617\u20103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010025\u201002155\u20104"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.930250"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010020\u201001159\u2010y"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010024\u201000982\u20102"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.929853"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201002095\u20106"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12860\u2010023\u201000466\u20105"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101753"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010023\u201001498\u20102"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1254313"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.70117"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2023.e01593"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2023"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007056\u20101"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201033711\u20100"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010023\u201001457\u20105"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00034.2"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c00770"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102795"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010024\u201000979\u20104"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010026\u201011487\u20106"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.5713\/ab.23.0014"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201080874\u20105"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201032101\u2010w"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418\u2010025\u201001997\u20101"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.08.018"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71555","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71555","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71555","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:26:41Z","timestamp":1788856001000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,31]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cbdv.71555"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71555","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,31]]},"assertion":[{"value":"2025-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71555"}}