{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:08:59Z","timestamp":1788858539858,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFE0205600"],"award-info":[{"award-number":["2020YFE0205600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YEE0104100"],"award-info":[{"award-number":["2025YEE0104100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diabetic hepatic injury is a common complication of diabetes, yet it is often neglected due to its subtle early symptoms. This study explored the effect and mechanism of\n                    <jats:italic>Anaphalis virgata<\/jats:italic>\n                    extract (AVE) in treating diabetic hepatic injury. Serum\u2010exposed components of AVE were identified by LC\u2010MS and used for network pharmacology to predict therapeutic targets and pathways. AVE was evaluated in diabetic rats treated intragastrically at 50, 100, and 200\u00a0mg\/kg for 10 weeks. Hepatic biochemical parameters were measured, and mechanisms were verified by molecular dynamics (MD) simulation, ELISA, and Western blot. Thirty\u2010one serum\u2010exposed compounds (8 prototypes and 23 metabolites) were identified, primarily flavonoids, phenolic acids, terpenoids, and phenylpropanoids. Network pharmacology revealed pathways including lipid and atherosclerosis, IL\u201017, and AGE\u2010RAGE signaling. Apigenin, kaempferol, and caffeic acid were identified as core compounds, and IL\u20106, TNF, and IL\u20101\u03b2 as core targets. In vivo, AVE significantly decreased hepatic IL\u20106, TNF, IL\u20101\u03b2, TC, TG, FFA, AST, and ALT levels in diabetic rats. Western blot showed that AVE up\u2010regulated AKT and AMPK while down\u2010regulating NF\u2010\u03baB and GSK\u20103\u03b2 pathways. AVE exerts a therapeutic effect on diabetic hepatic injury through anti\u2010inflammatory mechanisms, providing a foundation for its further development and clinical application.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71557","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T06:14:42Z","timestamp":1785824082000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploration of the Potential Mechanisms of\n                    <i>Anaphalis virgata<\/i>\n                    Extract in Treating Diabetic Hepatic Injury Based on Network Pharmacology and Experimental Validation"],"prefix":"10.1002","volume":"23","author":[{"given":"Wenyang","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences  Urumqi China"},{"name":"University of Chinese Academy of Sciences  Shijingshan District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences  Urumqi China"},{"name":"University of Chinese Academy of Sciences  Shijingshan District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4597-4047","authenticated-orcid":false,"given":"Sodik","family":"Numonov","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences  Urumqi China"},{"name":"Research Institution \u201cChinese\u2010Tajik Innovation Center for Natural Products\u201d of NAST  Dushanbe Tajikistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4652-6879","authenticated-orcid":false,"given":"Haji Akber","family":"Aisa","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences  Urumqi China"},{"name":"University of Chinese Academy of Sciences  Shijingshan District Beijing China"},{"name":"Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6009-2996","authenticated-orcid":false,"given":"Xuelei","family":"Xin","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry Chinese Academy of Sciences  Urumqi China"},{"name":"University of Chinese Academy of Sciences  Shijingshan District Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acvd.2015.01.009"},{"key":"e_1_2_10_3_1","article-title":"Insights into the Epidemiology, Pathogenesis, and Therapeutics of Nonalcoholic Fatty Liver Diseases","volume":"6","author":"Yu Y.","year":"2019","journal-title":"Advancement of Science"},{"key":"e_1_2_10_4_1","first-page":"1047","article-title":"Nobiletin Alleviates OLT\u2010Induced Liver Ischemia\u2010Reperfusion Injury by Inhibiting Kupffer Cell Activation Through the AKT\/GSK3\u03b2\/NF\u2010\u03baB Pathway in Rats","volume":"37","author":"Wang J. 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