{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:09:11Z","timestamp":1788858551691,"version":"build-2803163510"},"reference-count":82,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pajanelia longifolia<\/jats:italic>\n                    (Willd.) is a traditionally used medicinal plant with limited scientific evidence regarding its phytochemical composition and pharmacological properties. This study aimed to characterize the phytochemical constituents of\n                    <jats:italic>P. longifolia<\/jats:italic>\n                    leaves and to evaluate their antioxidant, hypoglycemic, antidiarrheal, and analgesic potentials using the DPPH radical\u2010scavenging assay, oral glucose tolerance test, castor oil\u2010induced diarrhea, tail immersion, and acetic acid\u2010induced writhing models, respectively. Methanolic leaf extract was subjected to GC\u2013MS\/MS analysis for phytochemical profiling. Molecular docking, drug\u2010likeness, pharmacokinetic, and toxicity analyses were performed to explore potential interactions between identified phytochemicals and target proteins. GC\u2013MS\/MS analysis identified 76 phytochemical constituents, including \u03b3\u2010sitosterol, squalene, loliolide, and various fatty acid derivatives. Among the tested fractions, the chloroform fraction demonstrated the maximum hypoglycemic and antidiarrheal activities. In contrast, the n\u2010hexane and ethyl acetate fractions showed notable central and peripheral analgesic effects, respectively. The aqueous fraction exhibited the strongest antioxidant activity. Molecular docking revealed favorable interactions of several identified compounds, particularly \u03b3\u2010sitosterol and phthalazine derivatives, with human pancreatic \u03b1\u2010amylase, M1 muscarinic acetylcholine receptor, \u03bc\u2010opioid receptor, and cyclooxygenase\u20102. Drug\u2010likeness, ADMET, and QSAR analyses further supported the pharmacological relevance of selected compounds. The combined findings suggest that the plant is a promising source of bioactive molecules.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71574","type":"journal-article","created":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T10:50:55Z","timestamp":1786272655000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unfolding the Pharmacological Potentialities and Phytochemical Characterization of\n                    <i>Pajanelia longifolia<\/i>\n                    (Wild) Leaves: A Multidimensional Study via GC\u2010MS\/MS, In Vitro, In Vivo, and In Silico Approaches"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9247-809X","authenticated-orcid":false,"given":"Md. Rasul","family":"Karim","sequence":"first","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Alfaz","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md.","family":"Shamim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Riaz","family":"Hosen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Sabbir","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy University of Dhaka  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Tanvir","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mst. Sumaya","family":"Akhter","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadija Tul","family":"Simran","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Islamic University  Kushtia Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5792-463X","authenticated-orcid":false,"given":"Md. Saiful","family":"Islam","sequence":"additional","affiliation":[{"name":"Pharmaceutical Sciences Research Division, BCSIR Dhaka Laboratories Bangladesh Council of Scientific and Industrial Research (BCSIR)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,9]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101\u2010025\u201010194\u20107"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.5897\/IJBC11.163"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42269\u2010022\u201000770\u20108"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1347069"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.03.026"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.21746\/aps.2017.01.006"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02854894"},{"key":"e_1_2_14_9_1","first-page":"11","article-title":"Antioxidant and Antibacterial Properties of Pajanelia longifolia (Willd.) K. Schum","volume":"2","author":"Subrahmanya Padyana S. P.","year":"2011","journal-title":"Annals of Biological Research"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.14719\/pst.2021.8.3.1104"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nem096"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.4172\/2153\u20102435.1000e139"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.22159\/ijpps.2017v9i9.19075"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1753\u20100407.70014"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2020.105280"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2022.06"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241612676"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-443-30080-6.00010-9"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468\u20102667(24)00302\u20105"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010023\u201001398\u20105"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000003026"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11030909"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011\u2010022\u201001546\u20106"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202403028"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7697"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.4236\/ojepi.2021.113018"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.3897\/phytokeys.253.144408"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2026.04.008"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210\u2010025\u201004814\u20104"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.14739\/2310\u20101210.2022.1.241286"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2025_772"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.3329\/dujps.v12i2.17625"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210\u2010019\u201001742\u2010y"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.11.009"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.2147\/JEP.S441273"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12247\u2010026\u201010643\u20106"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.53552\/ijmfmap.11.1.2025.252\u2010263"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv951"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10040728"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology15080592"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.3329\/dujps.v24i1.82409"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2222\u20101808(12)60209\u20106"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.71364"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2012.09.021"},{"key":"e_1_2_14_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.proche.2014.12.007"},{"key":"e_1_2_14_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202204512"},{"key":"e_1_2_14_48_1","doi-asserted-by":"publisher","DOI":"10.22038\/IJBMS.2019.35595.8488"},{"key":"e_1_2_14_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24040740"},{"key":"e_1_2_14_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2021.174091"},{"key":"e_1_2_14_51_1","doi-asserted-by":"publisher","DOI":"10.24214\/jecet.B.7.2.15779"},{"key":"e_1_2_14_52_1","first-page":"585","article-title":"Molecular Docking, Validation, Dynamics Simulations, and Pharmacokinetic Prediction of Natural Compounds Against the SARS\u2010CoV\u20102 Main\u2010Protease","volume":"40","author":"Shivanika C.","year":"2020","journal-title":"Journal of Biomolecular Structure & Dynamics"},{"key":"e_1_2_14_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c05565"},{"key":"e_1_2_14_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmgm.2018.05.010"},{"key":"e_1_2_14_55_1","doi-asserted-by":"publisher","DOI":"10.31643\/2023\/6445.13"},{"key":"e_1_2_14_56_1","doi-asserted-by":"publisher","DOI":"10.55927\/ijcs.v1i1.7271"},{"key":"e_1_2_14_57_1","doi-asserted-by":"publisher","DOI":"10.2174\/0113816128373506250414160220"},{"key":"e_1_2_14_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521\u20103838(200012)19:6<599::AID\u2010QSAR599>3.0.CO;2\u2010B"},{"key":"e_1_2_14_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jscs.2015.11.002"},{"key":"e_1_2_14_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280\u2010018\u20103601\u20100"},{"key":"e_1_2_14_61_1","doi-asserted-by":"publisher","DOI":"10.1080\/03639045.2022.2122985"},{"key":"e_1_2_14_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.12.061"},{"key":"e_1_2_14_63_1","doi-asserted-by":"publisher","DOI":"10.21608\/ajps.2016.6930"},{"key":"e_1_2_14_64_1","first-page":"282","article-title":"Antibacterial and GC\u2010MS Analysis of Stem and Leaf of Premna paucinervis (CB Clarke) Gamble (Lamiaceae)\u2014An Endemic and Rediscovered Species","volume":"6","author":"Francis S.","year":"2021","journal-title":"International Journal of Botany Studies"},{"key":"e_1_2_14_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202501615"},{"key":"e_1_2_14_66_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf070952p"},{"issue":"44","key":"e_1_2_14_67_1","first-page":"1","article-title":"An Overview of the Mechanisms of Plant Ingredients in the Treatment of Diabetes Mellitus","volume":"11","author":"Bathaie S. Z.","year":"2012","journal-title":"Journal of Medicinal Plants"},{"key":"e_1_2_14_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.553"},{"key":"e_1_2_14_69_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15143266"},{"key":"e_1_2_14_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms11041365"},{"key":"e_1_2_14_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010017\u20101697\u20100"},{"key":"e_1_2_14_72_1","doi-asserted-by":"publisher","DOI":"10.19082\/1832"},{"key":"e_1_2_14_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1907"},{"key":"e_1_2_14_74_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00316.2021"},{"key":"e_1_2_14_75_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002347"},{"key":"e_1_2_14_76_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7138"},{"key":"e_1_2_14_77_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202502470"},{"key":"e_1_2_14_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1995\u20107645(11)60180\u20106"},{"key":"e_1_2_14_79_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxres\/tfaa023"},{"key":"e_1_2_14_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202503467"},{"key":"e_1_2_14_81_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/340315"},{"key":"e_1_2_14_82_1","doi-asserted-by":"publisher","DOI":"10.2174\/1386207321666180213092018"},{"key":"e_1_2_14_83_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161206779010431"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71574","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71574","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71574","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:29:18Z","timestamp":1788856158000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":82,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cbdv.71574"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71574","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71574"}}