{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:09:22Z","timestamp":1788858562350,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Fraxinus angustifolia<\/jats:italic>\n                    Vahl. has long been used in traditional medicine for the management of inflammatory and gastrointestinal disorders. This study investigated the anti\u2010urease, anti\u2010\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    , and anti\u2010inflammatory activities of the ethanolic leaf extract and its phenolic fractions, with a focus on the mechanisms underlying these effects. The extracts exhibited inhibition of urease activity and bacterial growth, with the aqueous fraction of chloroform (AC) showing the most pronounced effect (urease IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a0384.55\u00a0\u00b1\u00a04.31\u00a0\u00b5g\/mL; MIC\u00a0=\u00a01000\u00a0\u00b5g\/mL against\n                    <jats:italic>H. pylori<\/jats:italic>\n                    ). HPLC\u2010DAD analysis identified 11 phenolic compounds, including flavones and flavonols. Quercetin\u20103\u2010O\u2010glucoside (3585.25\u00a0\u00b5g\/g) and rutin (936.11\u00a0\u00b5g\/g), were the most abundant phenolic compounds in the crude extract (CE), whereas chlorogenic acid (824.51\u00a0\u00b5g\/g), rosmarinic acid (586.47\u00a0\u00b5g\/g), and apigenin (148.25\u00a0\u00b5g\/g) predominated in the AC fraction. Enzyme kinetic analysis revealed a mixed mode of urease inhibition, while molecular docking predicted stable binding of phenolic compounds within the urease catalytic pocket and adjacent regions. These findings provide new evidence supporting\n                    <jats:italic>F. angustifolia<\/jats:italic>\n                    leaves as a relevant source of urease inhibitors, suggesting its potential for the management of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection and related gastric disorders. Further studies are underway to isolate the active compounds and evaluate their in vivo efficacy.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71583","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:08:10Z","timestamp":1786720090000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Profiling, Biological Evaluation, and Molecular Insights Into the Urease Inhibitory Potential of\n                    <i>Fraxinus angustifolia<\/i>\n                    Crude Extract and Fractions"],"prefix":"10.1002","volume":"23","author":[{"given":"Sarra","family":"Belkhir","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia Bejaia 06000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Rahmani\u2010Berboucha","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia Bejaia 06000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0769-1823","authenticated-orcid":false,"given":"Sarah Sihem","family":"Zemam","sequence":"additional","affiliation":[{"name":"Laboratory of Enterobacteria and Other Related Bacteria Pasteur Institute of Algeria Algiers 16000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadjet","family":"Debbache\u2010Benaida","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia Bejaia 06000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma Duygu","family":"Ceylan","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering Istanbul Technical University Istanbul 34469 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3723-3003","authenticated-orcid":false,"given":"Nabil","family":"Adrar","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering Istanbul Technical University Istanbul 34469 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amina","family":"Atia","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia Bejaia 06000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naima","family":"Saidene","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia Bejaia 06000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabila","family":"Benamrouche","sequence":"additional","affiliation":[{"name":"Laboratory of Enterobacteria and Other Related Bacteria Pasteur Institute of Algeria Algiers 16000 Algeria"},{"name":"Faculty of Medicine University of Health Sciences Algiers 16000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina","family":"Atmani\u2010Kilani","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie Appliqu\u00e9e, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia Bejaia 06000 Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esra","family":"Capanoglu","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering Istanbul Technical University Istanbul 34469 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Institut Pasteur \u201cStomach cancer and gastric ulcers \u201dInstitut Pasteur 2025 https:\/\/www.pasteur.fr\/en\/medical\u2010center\/disease\u2010sheets\/stomach\u2010cancer\u2010and\u2010gastric\u2010ulcers(accessed 11 August 2025)."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01883"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2015.06.002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.811258"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2017.02.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13497"},{"key":"e_1_2_9_8_1","volume-title":"WHO Global Report on Traditional and Complementary Medicine","author":"World Health 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