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However, no in vivo evidence of this effect has been found yet. Therefore, the aim is to evaluate its possible interaction in the Ras pathway using the nematode\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    and computational models. For the in vivo assays, we used a strain with a gain\u2010of\u2010function in the\n                    <jats:italic>let\u201060<\/jats:italic>\n                    gene, which is a human Ras homologous protein that leads to the formation of tumorous multivulva. The experimental tests indicated that a single 30\u00a0min treatment with ULI B was safe regarding toxicological parameters, and the worms demonstrated a delay in the multivulva phenotype development (MV) on day 1 and 2 of adulthood, with statistical significance at 5, 10, and 20\u00a0\u00b5M. In silico, ULI B showed a possible allosteric and non\u2010competitive interaction with LET\u201060, but not as significant as in the human Ras, possibly justifying the weak effect found with the delay in MV development in the nematode. However, based on the in\u2010silico data, ULI B seems promising against human cancer cells, as its interactions with EGFR and HRAS were more favorable than those with\n                    <jats:italic>C. elegans<\/jats:italic>\n                    proteins.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71592","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:47:06Z","timestamp":1786988826000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Uliginosin B as a Modulator of the Ras Pathway: In Vivo Evidence From\n                    <i>Caenorhabditis elegans<\/i>\n                    and In Silico Insights"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6232-6594","authenticated-orcid":false,"given":"Maria Eduarda O. De","family":"Souza","sequence":"first","affiliation":[{"name":"Research Group on Biochemistry and Toxicology in Caenorhabditis elegans Graduation Program in Biochemistry Federal University of Pampa  Uruguaiana Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0286-1424","authenticated-orcid":false,"given":"Fl\u00e1via Suelen De O.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Northwest Regional University of the State of Rio Grande Do Sul  Iju\u00ed Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Teixeira","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Graduation Program in Biological Sciences \u2010 Toxicological Biochemistry Federal University of Santa Maria  Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7294-9958","authenticated-orcid":false,"given":"Helder Dias","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Research and Drugs Development Graduation Program in Pharmaceutical Sciences Federal University of Pampa  Uruguaiana Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarsila Dantas Da","family":"Rosa","sequence":"additional","affiliation":[{"name":"Research Group on Biochemistry and Toxicology in Caenorhabditis elegans Graduation Program in Biochemistry Federal University of Pampa  Uruguaiana Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Machado Pires da","family":"Silva","sequence":"additional","affiliation":[{"name":"Integrated Regional University of Alto Uruguai and Miss\u00f5es  Frederico Westphalen Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2527-7500","authenticated-orcid":false,"given":"Gilsane von","family":"Poser","sequence":"additional","affiliation":[{"name":"Graduation Program in Pharmaceutical Sciences Federal University of Rio Grande Do Sul  Porto Alegre Rio Grande do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7952-7830","authenticated-orcid":false,"given":"Stela M. 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