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This study aimed to develop and characterize nanoformulations containing essential oils from\n                    <jats:italic>Piper<\/jats:italic>\n                    spp., and to evaluate their safety and efficacy in cell lines, as well as their antimicrobial potential against pathogenic microorganisms. To prepare the nanoemulsions, the essential oils were mixed with Tween 80 in varying ratios (1:1, 1:1.5, 1:2, and 1:2.5). The stability of the nanoformulations was monitored through visual inspection and measurements of particle size, zeta potential, and pH, along with characterization by transmission electron microscopy. Cytotoxic and antimicrobial activities were assessed using colorimetric assays with breast cancer cells (MDA\u2010MB\u2010231) and Vero cells (CCL81), as well as pathogenic bacteria and fungi such as\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    spp.,\n                    <jats:italic>Candida<\/jats:italic>\n                    spp., and\n                    <jats:italic>Penicillium<\/jats:italic>\n                    sp. The\n                    <jats:italic>Piper aduncum<\/jats:italic>\n                    nanoemulsion was the most promising sample, eliminating essential oil toxicity to healthy cells (IC50 &gt;100\u00a0\u00b5g\/mL) and exhibiting the strongest antifungal activity, with MIC values of 125\u00a0\u00b5g\/mL against\n                    <jats:italic>Penicillium adametzii<\/jats:italic>\n                    and 250\u00a0\u00b5g\/mL against\n                    <jats:italic>Aspergillus flavus<\/jats:italic>\n                    , compared to 1000\u00a0\u00b5g\/mL for the free essential oil. These nanoemulsions remained stable for up to 360 days and had particle sizes ranging from 26.4 \u00b1 0.42\u00a0to 265 \u00b1 0.00\u00a0nm with zeta potentials between \u221224.95 \u00b1 0.92\u00a0mV and \u221255.65 \u00b1 0.21\u00a0mV. This study demonstrates that nanoemulsions derived from\n                    <jats:italic>Piper<\/jats:italic>\n                    spp. are promising candidates against pathogenic microorganisms, with potentials applications in health and food preservation.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71614","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:30:06Z","timestamp":1787754606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nanoemulsions With Essential Oils of\n                    <i>Piper<\/i>\n                    spp. and Their Antifungal and Cytotoxic Potential"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1550-7766","authenticated-orcid":false,"given":"Midi\u00e3 Rodrigues de","family":"Oliveira","sequence":"first","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9614-3409","authenticated-orcid":false,"given":"Roosalyn Santos da","family":"Silva","sequence":"additional","affiliation":[{"name":"Amazonas State University  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0241-2766","authenticated-orcid":false,"given":"Brendo Pablo Ribeiro da","family":"Silva","sequence":"additional","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8279-5470","authenticated-orcid":false,"given":"Marcelo","family":"Sousa","sequence":"additional","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3003-7280","authenticated-orcid":false,"given":"Henrique Alves da","family":"Silva","sequence":"additional","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4940-9555","authenticated-orcid":false,"given":"Lizandro","family":"Manzato","sequence":"additional","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9001-7954","authenticated-orcid":false,"given":"Gemilson","family":"Soares Pontes","sequence":"additional","affiliation":[{"name":"National Institute of Amazonian Research  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5602-8617","authenticated-orcid":false,"given":"Antonia Queiroz Lima de","family":"Souza","sequence":"additional","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1119-7238","authenticated-orcid":false,"given":"Rita de C\u00e1ssia Saraiva","family":"Nunomura","sequence":"additional","affiliation":[{"name":"Federal University of Amazonas  Manaus Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.37349\/eds.2024.00054"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13050"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2011.140685"},{"key":"e_1_2_10_5_1","unstructured":"WHO World Health Organization. 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