{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:09:02Z","timestamp":1788858542826,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Inflammatory pain mediated by pro\u2010inflammatory factors remains a major clinical challenge, highlighting the need for safer alternatives to conventional anti\u2010inflammatory drugs. Here, the antinociceptive effects of methanolic extracts from the aerial parts of\n                    <jats:italic>Alchemilla daghestanica<\/jats:italic>\n                    (AD) and\n                    <jats:italic>Alchemilla minusculiflora<\/jats:italic>\n                    (AM) were evaluated in mice using the formalin test, and anti\u2010inflammatory activity was assessed by measuring formalin\u2010induced paw edema. Phytochemical characterization was performed by LC\u2013MS\/MS, and potential activities of major constituents were predicted using PASS Online. LC\u2013MS\/MS identified miquelianin as the predominant compound in both extracts. AD (100\u2013200\u00a0mg\/kg) and AM (200\u00a0mg\/kg) significantly inhibited the inflammatory phase of the formalin test (\n                    <jats:italic>p &lt;<\/jats:italic>\n                    0.001 and\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.01, respectively) without affecting the neurogenic phase, and AM extract reduced paw edema (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). PASS predicted high probabilities (Pa &gt; 0.7) for anti\u2010inflammatory activity of miquelianin, isoquercitrin, and tannic acid. These findings suggest that these extracts may represent valuable candidates for further investigation in inflammatory pain management.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71626","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:01:06Z","timestamp":1787752866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antinociceptive and Anti\u2010Inflammatory Effects of\n                    <i>Alchemilla daghestanica<\/i>\n                    and\n                    <i>Alchemilla minusculiflora<\/i>\n                    : Quantitative Phytochemical Profiling and In Vivo Evidence"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3284-848X","authenticated-orcid":false,"given":"Elif Nur","family":"Barut","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy Department of Pharmacology Karadeniz Technical University  Trabzon 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