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In this study, an undescribed pyrazine derivative 5\u2010isobutyryl\u20101,4\u2010dihydropyrazine\u20102,3\u2010dione (\n                    <jats:bold>1<\/jats:bold>\n                    ) together with three known compounds (\n                    <jats:bold>2\u2010<\/jats:bold>\n                    \u2010\n                    <jats:bold>4<\/jats:bold>\n                    ) were isolated from the marine\u2010derived fungus\n                    <jats:italic>Aspergillus ochraceus<\/jats:italic>\n                    LSD\u201013. Compound\n                    <jats:bold>2<\/jats:bold>\n                    was a potent virulence attenuator of\n                    <jats:italic>Chromobacterium violaceum<\/jats:italic>\n                    CV026 with a minimum inhibitory concentration (MIC) of 32 \u00b5g\/mL. At sub\u2010MIC, compound\n                    <jats:bold>2<\/jats:bold>\n                    significantly suppressed the production of QS\u2010controlled phenotypes: violacein,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acyl homoserine lactone, chitinase, and extracellular polysaccharide. It also reduced biofilm formation by 73.3% at 1\/2 MIC, a phenotype confirmed by scanning electron microscopy. Furthermore, compound\n                    <jats:bold>2<\/jats:bold>\n                    inhibited swarming motility in a dose\u2010dependent manner. Mechanistic investigations revealed that compound\n                    <jats:bold>2<\/jats:bold>\n                    downregulated the expression of QS\u2010associated genes (\n                    <jats:italic>cviR<\/jats:italic>\n                    ,\n                    <jats:italic>vioA<\/jats:italic>\n                    ,\n                    <jats:italic>vioC<\/jats:italic>\n                    ). Molecular docking demonstrated that compound\n                    <jats:bold>2<\/jats:bold>\n                    has a strong binding affinity for CviR protein with \u22126.8\u00a0kcal\/mol, suggesting a competitive antagonism of the native C\n                    <jats:sub>6<\/jats:sub>\n                    HSL ligand. These findings highlight compound\n                    <jats:bold>2<\/jats:bold>\n                    as a promising QS inhibitor with therapeutic potential against\n                    <jats:italic>C. violaceum<\/jats:italic>\n                    CV026.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71640","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:37:54Z","timestamp":1787755074000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of Virulence Attenuator of\n                    <i>Chromobacterium violaceum<\/i>\n                    CV026 From Marine\u2010Derived Fungus\n                    <i>Aspergillus ochraceus<\/i>\n                    LSD\u201013 by Inhibiting Quorum Sensing and Destroying Biofilm"],"prefix":"10.1002","volume":"23","author":[{"given":"Xinyi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibo","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shurong","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwei","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0852-4047","authenticated-orcid":false,"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Natural Medicine and Pharmacognosy School of Pharmacy Qingdao University  Qingdao P. R. 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