{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:58:03Z","timestamp":1790542683672,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Natural products rich in phenolic compounds are increasingly investigated as multifunctional agents against oxidative stress and enzyme\u2010related disorders. This study characterized the phytochemical composition and evaluated the antioxidant and enzyme inhibitory activities of methanol and aqueous extracts of\n                    <jats:italic>Stachys aleurites<\/jats:italic>\n                    . Total phenolic and flavonoid contents were determined spectrophotometrically, while selected phenolics were quantified by LC\u2010ESI\u2010MS\/MS. Antioxidant and enzyme inhibitory properties were assessed using complementary in vitro assays.\n                  <\/jats:p>\n                  <jats:p>\n                    The methanol extract contained higher levels of total phenolics (48.86\u00a0mg GAE\/g) and flavonoids (65.36\u00a0mg RE\/g) than the aqueous extract. Verbascoside and chlorogenic acid were the predominant compounds in the methanol extract, whereas\n                    <jats:italic>p<\/jats:italic>\n                    \u2010coumaric acid was relatively more abundant in the aqueous extract. The methanol extract exhibited stronger antioxidant activity in most assays and showed greater inhibition of AChE, tyrosinase, \u03b1\u2010amylase, and \u03b1\u2010glucosidase. In contrast, the aqueous extract displayed slightly higher metal\u2010chelating activity and stronger BChE inhibition. These findings demonstrate solvent\u2010dependent variations in phytochemical composition and bioactivity, highlighting the importance of extraction solvent selection and supporting further studies on the bioactive constituents of\n                    <jats:italic>S. aleurites<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71679","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:21:12Z","timestamp":1788524472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenolic Profiling and Bioactivity Evaluation of\n                    <i>Stachys aleurites<\/i>\n                    Extracts: LC\u2013ESI\u2013MS\/MS Characterization, Antioxidant Capacity, and Enzyme Inhibitory Potential"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3908-3371","authenticated-orcid":false,"given":"Fatma Ozlem Kargin","family":"Solmaz","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy Afyonkarahisar Health Sciences University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5094-2520","authenticated-orcid":false,"given":"Cengiz","family":"Sarikurkcu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Afyonkarahisar Health Sciences University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8982-5188","authenticated-orcid":false,"given":"Bekta\u015f","family":"Tepe","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Molecular Biology and Genetics Kilis 7 Aralik University  Kilis T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201044337\u20107"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.25135\/rnp.529.2505.3521"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28041845"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines7100063"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29225345"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.47947\/ijnls.736452"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bse.2004.05.013"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10600\u2010006\u20100070\u20101"},{"key":"e_1_2_12_10_1","first-page":"154","article-title":"Antimicrobial Activity of Centaura Derderiifolia, Stachys aleurites and Anthemis aciphylla","volume":"3","author":"G\u00fcven K.","year":"2009","journal-title":"Journal of Applied Biological Sciences"},{"key":"e_1_2_12_11_1","first-page":"17","article-title":"Fatty Acids and Tocopherols of Turkish Salvia Fruticose, Salvia tomentosa, Stachys aleurites and Stachys cretica subsp. anatolica Seed Oils","volume":"95","author":"\u00d6zkan G.","year":"2018","journal-title":"Rivista Italiana Delle Sostanze Grasse"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.12.012"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.11.009"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2022.05.046"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bse.2022.104482"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2016.1233410"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2020.101711"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12111644"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eujim.2020.101134"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2023.04.015"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1924265"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111639"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2025.2486414"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.26650\/IstanbulJPharm.2022.1075293"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.21448\/ijsm.1721228"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.04.023"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068\u2010010\u20100097\u20108"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmy003"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules31081240"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202504699"},{"key":"e_1_2_12_31_1","first-page":"1698","article-title":"Chemical Composition, Antimicrobial and Antioxidant Activity of Essential Oil of Endemic Ferula lycia Boiss","volume":"4","author":"Kose E. O.","year":"2010","journal-title":"Journal of Medicinal Plants Research"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111878"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2020.112448"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/09637480600798132"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules31050840"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71679","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71679","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:55:47Z","timestamp":1790542547000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":34,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/cbdv.71679"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71679","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71679"}}