{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:58:26Z","timestamp":1790542706117,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Peptic ulcer disease (PUD) continues to be an important gastrointestinal condition attributed to\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    infection, usage of nonsteroidal anti\u2010inflammatory drugs, oxidative stress, and inflammatory mediators. Although there are available treatments for this condition, side effects, relapse, and safety issues call for development of new multi\u2010target anti\u2010ulcer compounds. In this research, a group of benzimidazole\u2010pyrazole analogs (5a\u20135j) was designed, synthesized, and studied with the aid of computational and experimental methods. Molecular docking into H\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase (PDB ID: 5YLU) and COX\u20102 (PDB ID: 3LN1) enzymes predicted high affinity of these compounds with docking scores of \u22129.4 and \u22129.3\u00a0kcal\/mol for compounds 5i and 5e, respectively, higher than those obtained for the reference drugs omeprazole and celecoxib. The inhibition of the gastric H\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase enzyme was also observed in vitro, with compounds 5i and 5e having better inhibitory effects. Studies on the ethanol and indomethacin\u2010induced gastric ulceration models demonstrated good inhibitory effects of these compounds. Biochemical analysis showed that these analogs significantly decreased the levels of TNF\u2010\u03b1, COX\u20102, myeloperoxidase, and malondialdehyde and increased prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    . Acute oral toxicity studies indicated no observable toxicity at 100\u00a0mg\/kg. Collectively, these findings identify compounds 5i and 5e as promising multifunctional gastroprotective candidates warranting further pharmacological investigation.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71698","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:22:35Z","timestamp":1789467755000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Computational, In Vitro, and In Vivo Evaluation of Benzimidazole\u2013Pyrazole Hybrid Scaffolds as Multi\u2010Target Anti\u2010Ulcer Agents"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7365-2937","authenticated-orcid":false,"given":"Hafiz Aamir Ali","family":"Kharl","sequence":"first","affiliation":[{"name":"Riphah Institute of Pharmaceutical Sciences Riphah International University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Malik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0169-0811","authenticated-orcid":false,"given":"Humaira","family":"Nadeem","sequence":"additional","affiliation":[{"name":"Riphah Institute of Pharmaceutical Sciences Riphah International University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7479-8970","authenticated-orcid":false,"given":"Mohammad Shamsul","family":"Ola","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4115-4984","authenticated-orcid":false,"given":"Sara","family":"Aiman","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, Dongguan Affiliated Hospital Guangdong Medical University  Dongguan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arif Ullah","family":"Khan","sequence":"additional","affiliation":[{"name":"Riphah Institute of Pharmaceutical Sciences Riphah International University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Akmal","family":"Farooq","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Health &amp; Pharmaceutical Sciences University of Agriculture  Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2172-8474","authenticated-orcid":false,"given":"Humaira","family":"Muzaffar","sequence":"additional","affiliation":[{"name":"Department of Physiology Government College University Faisalabad  Faisalabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4401-6971","authenticated-orcid":false,"given":"Syed Muzzammil","family":"Masaud","sequence":"additional","affiliation":[{"name":"Department of Allied Health Sciences The Saifee Burhani University  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0325821"},{"key":"e_1_2_11_3_1","volume-title":"Burger's Medicinal Chemistry & Drug Discovery","author":"Herling A. 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