{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:58:02Z","timestamp":1790542682808,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008366","name":"Guangdong Pharmaceutical University","doi-asserted-by":"publisher","award":["S202410573004"],"award-info":[{"award-number":["S202410573004"]}],"id":[{"id":"10.13039\/501100008366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Although\n                    <jats:italic>Arctium lappa<\/jats:italic>\n                    L. polysaccharides (ALPs) possess recognized pharmacological potential, their green extraction using deep eutectic solvents (DESs) combined with ultrasound has not been previously explored. This study reports, for the first time, an optimized DES\u2010based ultrasound\u2010assisted extraction (DES\u2010UAE) protocol for ALPs. Response surface optimization identified the following conditions: choline chloride\/oxalic acid molar ratio of 2:1, water content of 18.508%, liquid\u2010to\u2010solid ratio of 37.425\u00a0mL\/g, extraction time of 97.262\u00a0min, and ultrasonic power of 275.787\u00a0W, yielding 16.581% polysaccharides. Sequential chromatographic purification afforded a homogeneous fraction, ALPs\u20101, with a weight\u2010average molecular weight of 8827\u00a0Da. Monosaccharide analysis revealed that ALPs\u20101 is composed of rhamnose, glucuronic acid, glucose, and galactose at a molar ratio of 2.18:1.00:6.62:7.17. Functionally, ALPs\u20101 exhibited moderate \u03b1\u2010glucosidase inhibition, weak DPPH\u2022 scavenging, and notable ABTS\u2022\n                    <jats:sup>+<\/jats:sup>\n                    scavenging (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a00.87\u00a0mg\/mL). Importantly, ALPs\u20101 significantly suppressed NO, TNF\u2010\u03b1, and IL\u20101\u03b2 production in LPS\u2010stimulated BV2 microglia and protected zebrafish keratinocytes from metronidazole\u2010induced oxidative injury. These results demonstrate that ALPs\u20101 possesses promising antioxidant, hypoglycemic, and anti\u2010neuroinflammatory activities. This work provides the first evidence for the effective application of DES\u2010UAE in extracting bioactive ALPs, supporting their potential use in functional foods and pharmaceuticals.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71714","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:39:41Z","timestamp":1790141981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasonic\u2010Assisted Deep Eutectic Solvent Extraction, Characterization, and Bioactivities of\n                    <i>Arctium lappa<\/i>\n                    L. Polysaccharides"],"prefix":"10.1002","volume":"23","author":[{"given":"Hongxin","family":"Zhou","sequence":"first","affiliation":[{"name":"Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikai","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Pharmaceutical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YingTing","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Pharmaceutical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4673-9068","authenticated-orcid":false,"given":"Haiyun","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Pharmaceutical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Fan","sequence":"additional","affiliation":[{"name":"Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0690-1673","authenticated-orcid":false,"given":"Zaijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Pharmaceutical University  Guangzhou China"},{"name":"School of Pharmacy Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9274-2156","authenticated-orcid":false,"given":"Yao","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Pharmaceutical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.109889"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2017.12.009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.07.040"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2022.116798"},{"key":"e_1_2_10_6_1","first-page":"260","article-title":"Study on Extraction of Burdock Polysaccharides by Double\u2010Enzyme Method","volume":"29","author":"Gao M.","year":"2008","journal-title":"Food Science"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2014.01.062"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.11.087"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.06.266"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.131035"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.10.061"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2023.101166"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.963318"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2023.106295"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2022.105966"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27123909"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2023.102852"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.117729"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.12.134"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.03.029"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2019.115405"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2019.05.004"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.116132"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2010.05.029"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2023.101168"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2022.105289"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2022.100877"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10102330"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.131636"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2012.03.008"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2022.100855"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2023.106474"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.03.083"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.12.096"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.134118"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30142943"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71714","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71714","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71714","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:55:48Z","timestamp":1790542548000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71714"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":35,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/cbdv.71714"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71714","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71714"}}