{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:58:43Z","timestamp":1790542723871,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Traditional Chinese medicines (TCMs) are rich sources of structurally diverse bioactive compounds, yet rapid identification of protease inhibitors from complex botanical matrices remains challenging. Here, a magnetic bead\u2010based affinity\u2010fishing platform coupled with UPLC\u2010Q\u2010Exactive\u2010Orbitrap\u2010MS\/MS was applied to identify SARS\u2010CoV\u20102 main protease (M\n                    <jats:sup>pro<\/jats:sup>\n                    ) inhibitors from\n                    <jats:italic>Reynoutria japonica<\/jats:italic>\n                    . The crude ethanolic extract inhibited M\n                    <jats:sup>pro<\/jats:sup>\n                    with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 44.92\u00a0\u00b1\u00a02.31\u00a0\u00b5g\/mL. M\n                    <jats:sup>pro<\/jats:sup>\n                    \u2010functionalized magnetic beads enriched eight constituents, which were identified by high\u2010resolution mass spectrometry and evaluated using a FRET\u2010based inhibition assay. Six compounds showed concentration\u2010dependent activity, with resveratrol being the most potent (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a034.01\u00a0\u00b1\u00a01.67\u00a0\u00b5mol\/L), followed by glycitein, genistein, emodin, apigenin\u20107\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glucoside, and biochanin A. HPLC\u2010DAD further quantified the principal active constituents. Molecular docking suggested interactions with residues in the Mpro substrate\u2010binding pocket, while network pharmacology predicted associations with host\u2010related targets involving IL6, TNF, and AKT1. Overall, this workflow enables efficient enrichment, structural identification, and functional prioritization of M\n                    <jats:sup>pro<\/jats:sup>\n                    \u2010inhibitory constituents from complex botanical extracts, providing a practical strategy for target\u2010directed natural product discovery.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71722","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:06:35Z","timestamp":1790539595000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Magnetic Bead\u2010Based Ligand Fishing Identifies SARS\u2010CoV\u20102 Main Protease Inhibitors From\n                    <i>Reynoutria japonica<\/i>"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9459-7378","authenticated-orcid":false,"given":"Yujing","family":"Huang","sequence":"first","affiliation":[{"name":"School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou P.R. China"},{"name":"College of Pharmacy Jinan University  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenjing","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlian","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duntao","family":"Zu","sequence":"additional","affiliation":[{"name":"School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianni","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Jinan University  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanrong","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Affiliated Panyu Central Hospital of Guangzhou Medical University  Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Ren","sequence":"additional","affiliation":[{"name":"Guangdong Second Provincial General Hospital Internet Medical Center Guangzhou P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1107-6284","authenticated-orcid":false,"given":"Junxia","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou P.R. 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