{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T21:56:24Z","timestamp":1790546184648,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study investigated the fatty acid composition of\n                    <jats:italic>Linum strictum<\/jats:italic>\n                    seed oil and evaluated its antibacterial, antibiofilm, anticoagulant, and \u03b1\u2010amylase inhibitory activities. The oil was extracted from commercially obtained seeds and characterized by GC\u2010FID. Antibacterial activity was determined using the microbroth dilution method, while antibiofilm activity was assessed against multidrug\u2010resistant strains, including\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA), and\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    . Anticoagulant effects were evaluated using prothrombin time (PT) and activated partial thromboplastin time (aPTT), and \u03b1\u2010amylase inhibition was assessed in vitro. The oil was characterized by a high proportion of unsaturated fatty acids, mainly \u03b1\u2010linolenic (57.08%), oleic (17.53%), and linoleic (14.0%) acids. Significant antibacterial activity was observed against\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    ATCC 29212 and\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    A2553 and A722 (MIC\u00a0=\u00a01.25\u00a0mg\/mL), while three\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains showed MIC values of 5\u00a0mg\/mL. Biofilm inhibition exceeded 70% against\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    at 2\u00d7MIC. The oil prolonged coagulation times, particularly through the intrinsic pathway (PT\u00a0=\u00a016.4\u00a0s; aPTT\u00a0=\u00a081.6\u00a0s). \u03b1\u2010Amylase inhibition reached 85.98% (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a00.0134\u00a0mg\/mL), comparable to acarbose. Molecular docking revealed favorable binding of \u03b1\u2010oleic acid to BEL\u20101 \u03b2\u2010lactamase and \u03b1\u2010linolenic acid to DNA gyrase B.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71743","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:20:38Z","timestamp":1789971638000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fatty Acid Composition of Fixed Oil of\n                    <i>Linum strictum<\/i>\n                    and Its Antibiofilm, Anticoagulant, and \u03b1\u2010Amylase Inhibitory Effects\u2013In Vitro and In Silico Study"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2905-8129","authenticated-orcid":false,"given":"Hayet","family":"Edziri","sequence":"first","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy University of Monastir  Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3419-5119","authenticated-orcid":false,"given":"Pooja","family":"Bargali","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Basic Sciences and Humanities G.B. Pant University of Agriculture and Technology  U.S. Nagar Uttarakhand India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4858-2172","authenticated-orcid":false,"given":"Marwa","family":"Melliti","sequence":"additional","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy University of Monastir  Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan","family":"Aljohi","sequence":"additional","affiliation":[{"name":"Applied Genetics Technology Institute King Abdulaziz City for Science and Technology  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1520-9941","authenticated-orcid":false,"given":"Mohammad","family":"NasserAlkhrayef","sequence":"additional","affiliation":[{"name":"Disability Research Institute King Abdulaziz City For Science and Technology  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rim","family":"Gtari","sequence":"additional","affiliation":[{"name":"Laboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy University of Monastir  Monastir Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5063-1236","authenticated-orcid":false,"given":"Marcello","family":"Iriti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Surgical and Dental Sciences University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0296-5231","authenticated-orcid":false,"given":"Ravendra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Basic Sciences and Humanities G.B. 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