{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:56:37Z","timestamp":1790794597143,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572709"],"award-info":[{"award-number":["32572709"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008250","name":"Shaanxi University of Science and Technology","doi-asserted-by":"publisher","award":["2022BJ\u201031"],"award-info":[{"award-number":["2022BJ\u201031"]}],"id":[{"id":"10.13039\/501100008250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To address the challenge of fungicide resistance in plant pathogenic fungi, coumarin derivatives were designed. Two series of coumarin derivatives, including 3\u2010amide and thienocoumarin ether derivatives, were designed and synthesized through structural modification of the coumarin scaffold to explore their antifungal potential. Their antifungal activities were evaluated using the mycelial growth rate method, with half\u2010maximal effective concentration (EC\n                    <jats:sub>50<\/jats:sub>\n                    ) values determined. The effects of compound\n                    <jats:bold>IVd<\/jats:bold>\n                    on membrane integrity and morphology were assessed by measuring electrolyte leakage, malondialdehyde (MDA) content, and scanning electron microscopy (SEM). Synergistic effects were quantified using Wadley's method by calculating the synergistic ratio (SR). Molecular docking studies were conducted to investigate interactions with potential targets (CYP51, CYP51B, and SDH). Among the derivatives,\n                    <jats:bold>IVd<\/jats:bold>\n                    showed the most potent antifungal activity, with EC\n                    <jats:sub>50<\/jats:sub>\n                    values of 88.5 \u00b5g\/mL against\n                    <jats:italic>Valsa mali<\/jats:italic>\n                    , slightly surpassing azoxystrobin (EC\n                    <jats:sub>50<\/jats:sub>\n                    = 92.8 \u00b5g\/mL). Treatment with\n                    <jats:bold>IVd<\/jats:bold>\n                    caused membrane disruption and hyphal deformation. A 2:1 combination ratio of\n                    <jats:bold>IVd<\/jats:bold>\n                    with azoxystrobin or fluconazole demonstrated synergistic effects (SR &gt; 1.5). Molecular docking suggested favorable interactions with the investigated targets, supporting its multitarget activity. Overall, these findings highlight\n                    <jats:bold>IVd<\/jats:bold>\n                    as a promising antifungal scaffold for investigation and provide evidence for its potential use in combination strategies against plant pathogenic fungi.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71773","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:16:00Z","timestamp":1790792160000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coumarin\u2010Based Antifungal Leads: Design, Synthesis, Mechanistic Insights, and Synergistic Activity"],"prefix":"10.1002","volume":"23","author":[{"given":"Xiangna","family":"Chang","sequence":"first","affiliation":[{"name":"School of Food Science and Engineering Shaanxi University of Science &amp; Technology  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Du","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Shaanxi University of Science &amp; Technology  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Shaanxi University of Science &amp; Technology  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jundi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Shaanxi University of Science &amp; Technology  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof11040274"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01719\u201015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.7223"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29194739"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2014.10.027"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12258"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7110900"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8121930"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082485"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202200802"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202000763"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25136879"},{"issue":"1","key":"e_1_2_10_14_1","first-page":"1","article-title":"Chiral Anticoagulants Drugs Based on Coumarin","volume":"2","author":"Kraimi A.","year":"2021","journal-title":"Aditum Journal of Clinical and Biomedical Research"},{"issue":"3","key":"e_1_2_10_15_1","first-page":"103","article-title":"The Antioxidant and Anticoagulant Effects of Coumarin and Quercetin From Cinnamon Methanolic Extract, and the Assessment of Cinnamon Powder Effect on Plasma Parameters in Diabetes, and the Disinfectant Activity in Diabetic Patients","volume":"4","author":"Shahri M. 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