{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:58:17Z","timestamp":1790704697104,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Niosomes are promising nanocarriers for improving the stability, bioavailability, and delivery of plant\u2010derived bioactive compounds. This study aimed to prepare and physicochemically characterize niosomes loaded with\n                    <jats:italic>Ocimum basilicum<\/jats:italic>\n                    L. extract and evaluate their anticancer and metastasis\u2010related effects in human ovarian cancer SKOV3 cells. The niosomes were characterized using DLS, SEM, FTIR, and in vitro release analysis, showing a mean particle size of 256.2\u00a0\u00b1\u00a00.21\u00a0nm, PDI of 0.265\u00a0\u00b1\u00a00.007, zeta potential of \u221235.4\u00a0\u00b1\u00a00.3\u00a0mV, and encapsulation efficiency of 85.2\u00a0\u00b1\u00a00.6%. Cytotoxicity was assessed by MTT assay after 24, 48, and 72\u00a0h, and the 72\u00a0h IC\n                    <jats:sub>50<\/jats:sub>\n                    (398.24\u00a0\u00b5g\/mL) was used for subsequent analyses. The extract\u2010loaded niosomes exhibited significantly greater cytotoxicity than the free extract and empty niosomes, particularly after 72\u00a0h. qRT\u2010PCR revealed significant upregulation of Bax (3.46\u2010fold) and downregulation of Bcl2 (0.67\u2010fold), MMP2 (0.63\u2010fold), and MMP9 (0.64\u2010fold) (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). Flow cytometry confirmed enhanced apoptosis, with 27.8% apoptotic and 5.87% necrotic cells. Overall,\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    extract\u2010loaded niosomes increased apoptosis and modulated apoptosis\u2010 and metastasis\u2010associated genes, supporting their potential as nanocarriers for plant\u2010derived anticancer compounds.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71779","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:19:13Z","timestamp":1790702353000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preparation and Characterization of\n                    <i>Ocimum basilicum<\/i>\n                    Extract\u2010Loaded Niosomes and Evaluation of Their Cytotoxic and Pro\u2010Apoptotic Potential in SKOV3 Ovarian Cancer Cells"],"prefix":"10.1002","volume":"23","author":[{"given":"Zahra","family":"Mousavi","sequence":"first","affiliation":[{"name":"Department of Biology, ET.C. Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1635-743X","authenticated-orcid":false,"given":"Mahsa","family":"Kavousi","sequence":"additional","affiliation":[{"name":"Department of Biology, ET.C. Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahnaz","family":"Farahmand","sequence":"additional","affiliation":[{"name":"Department of Biology, ET.C. Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"4","key":"e_1_2_14_2_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.21037\/cco-20-34","article-title":"Epidemiology of Ovarian Cancer","volume":"9","author":"Gaona\u2010Luviano P.","year":"2020","journal-title":"Chinese Clinical Oncology"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.18502\/ijph.v52i4.12453"},{"key":"e_1_2_14_4_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.bpobgyn.2020.02.010","article-title":"Ovarian Cancer Screening: Current Status and Future Directions","volume":"65","author":"Nash Z.","year":"2020","journal-title":"Best Practice & Research Clinical Obstetrics & Gynaecology"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001856\u20107"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432\u2010020\u201003136\u20107"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.12391"},{"issue":"2","key":"e_1_2_14_8_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ogrm.2023.11.002","article-title":"Effects of Cancer Treatment on Reproductive Health","volume":"34","author":"Palinska\u2010Rudzka K.","year":"2024","journal-title":"Obstetrics, Gynaecology & Reproductive Medicine"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22010173"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24076834"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11203212"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14102375"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072605"},{"key":"e_1_2_14_14_1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.jff.2018.05.001","article-title":"Chemopreventive Effects of Polyphenol\u2010rich Extracts Against Cancer Invasiveness and Metastasis by Inhibition of Type IV Collagenases Expression and Activity","volume":"46","author":"Chojnacka K.","year":"2018","journal-title":"Journal of Functional Foods"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2021.112859"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23126806"},{"issue":"12","key":"e_1_2_14_17_1","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1007\/s00109-021-02136-5","article-title":"Endocrine Resistance in Breast Cancer: From Molecular Mechanisms to Therapeutic Strategies","volume":"99","author":"Saatci O.","year":"2021","journal-title":"Journal of Molecular Medicine"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033\u2010024\u201009807\u20109"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.34172\/apb.2023.087"},{"issue":"3","key":"e_1_2_14_20_1","doi-asserted-by":"crossref","first-page":"616","DOI":"10.5530\/ijpi.14.3.74","article-title":"Advancement and Characteristics of Non\u2010Ionic Surfactant Vesicles (Niosome) and Their Application for Analgesics","volume":"14","author":"Pandey P.","year":"2024","journal-title":"International Journal of Pharmaceutical Investigation"},{"key":"e_1_2_14_21_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jddst.2023.104820","article-title":"Green Extraction of Essential Oils From Pistacia Lentiscus Resins: Encapsulation Into Niosomes Showed Improved Preferential Cytotoxic and Apoptotic Effects Against Breast and Ovarian Cancer Cells","volume":"87","author":"Fahmy S. 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T.Beyna \u201cReview on the Ethnopharmacological Use of Medicinal Plants and Their Anticancer Activity From Preclinical to Clinical Trial \u201d Natural Product Communications 20 no. 4 (2025): 1934578\u00d7251333027.","DOI":"10.1177\/1934578X251333027"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71779","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71779","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:19:17Z","timestamp":1790702357000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":32,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/cbdv.71779"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71779","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71779"}}