{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T07:45:54Z","timestamp":1791791154050,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2104800"],"award-info":[{"award-number":["2022YFC2104800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ceramides are essential lipid components of the skin barrier, accounting for approximately 50% of intercellular lipids in the stratum corneum. Their deficiency is associated with various skin disorders, including atopic dermatitis and xerosis. This study aimed to enzymatically synthesize a novel phytoceramide from perilla seed oil (PSO\u2010Cer) and evaluate its multifaceted bioactivity, including skin barrier enhancement and anti\u2010aging effects, in comparison with oleic acid\u2010derived Ceramide IIIB. PSO\u2010Cer was synthesized enzymatically and characterized by HPLC\u2010CAD and LC\u2010QTOF. Its biological effects were assessed in human keratinocytes (HaCaT) and dermal fibroblasts\u2010adult (HDF\u2010a) through cytotoxicity assays, qPCR of genes related to barrier function and extracellular matrix (ECM) synthesis, western blot, and transcriptome sequencing. HPLC\u2010CAD confirmed PSO\u2010Cer was rich in polyunsaturated fatty acid chains, notably \u03b1\u2010linolenic acid (34.49%). Both PSO\u2010Cer and Ceramide IIIB were non\u2010cytotoxic up to 25 \u00b5g\/mL and significantly upregulated genes critical for epidermal barrier function (FLG, IVL, TGM1, CERS3, and ELOVL4) and ECM components (COL1A1, COL3A1, and ELN) in UVB\u2010irradiated fibroblasts. Transcriptome analysis revealed that while both ceramides shared common mechanisms, namely activation of mTOR signaling and sphingolipid metabolism, PSO\u2010Cer exhibited distinct properties, positioning it as a promising multifunctional active for advanced skincare formulations aimed at comprehensive skin health.<\/jats:p>","DOI":"10.1002\/cbdv.71808","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T06:57:58Z","timestamp":1791788278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enzymatic Synthesis of a Novel \u03b1\u2010Linolenic Acid\u2010Enriched Phytoceramide From Perilla Seed Oil and Its Multifunctional Bioactivity in Skin Barrier Repair and Anti\u2010Aging"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2038-1865","authenticated-orcid":false,"given":"Jumao","family":"Yuan","sequence":"first","affiliation":[{"name":"Peterson's Lab  Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering State Key Laboratory of Materials\u2010Oriented Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Borui","family":"Shi","sequence":"additional","affiliation":[{"name":"Peterson's Lab  Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Peterson's Lab  Shanghai P.R. China"},{"name":"Baudry Biotech. Co., Ltd  Nanjing Jiangsu P.R. China"},{"name":"Institute of Symbolcell Biotechnology  Nanjing Jiangsu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Emergency Ruijin Hospital, Shanghai Jiaotong University School of Medicine  Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7934-6706","authenticated-orcid":false,"given":"Nuo","family":"Shi","sequence":"additional","affiliation":[{"name":"Peterson's Lab  Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[{"name":"Peterson's Lab  Shanghai P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000441540"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022\u2010202X.2005.23668.x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/00128071\u2010200304020\u201000004"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10092386"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40257\u2010021\u201000619\u20102"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104419\u20106741\u20101_1"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr494"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl480"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1619"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.88"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.2147\/ccid.S143591"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.14905"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M500221\u2010JLR200"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000464337"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25040989"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.68.85"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.344"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2019.07.009"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.2c05598"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcat.2019.01.007"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.202000115"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/op0680136"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcat.2024.114006"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29225258"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/dermato4010002"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71808","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71808","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T06:58:00Z","timestamp":1791788280000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/cbdv.71808"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71808","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71808"}}