{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T07:47:26Z","timestamp":1791791246286,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012155","name":"National Institute for Medical Research Development","doi-asserted-by":"publisher","award":["4000293"],"award-info":[{"award-number":["4000293"]}],"id":[{"id":"10.13039\/501100012155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, we report the synthesis of 14 Biginelli adducts and the evaluation of their anticancer activity against three human cancer cell lines: MCF\u20107 (breast), SW480 (colon), and Hep\u2010G2 (liver), as well as the noncancerous Hek\u2010293 cell line. Among the synthesized derivatives, compound\n                    <jats:bold>8n<\/jats:bold>\n                    emerged as the most potent, inducing significant cytotoxicity and apoptosis in MCF\u20107 cells. Structure\u2013activity relationship (SAR) analysis showed that specific modifications, such as methyl and nitro substitutions, enhanced anticancer activity, whereas bulky substituents reduced potency. Further investigations confirmed that compound\n                    <jats:bold>8n<\/jats:bold>\n                    induces cell cycle arrest at the S and G2\/M phases, highlighting its antiproliferative mechanism. Molecular docking and molecular dynamics simulations suggest that compound\n                    <jats:bold>8n<\/jats:bold>\n                    could bind to Eg5, a potential regulator of cell division. However, this study did not include direct biochemical testing of the protein target (Eg5).\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71810","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T07:09:55Z","timestamp":1791788995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design and Synthesis of Novel Biginelli Adducts: Anticancer Activity and In Silico Study"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8442-2205","authenticated-orcid":false,"given":"Aida","family":"Iraji","sequence":"first","affiliation":[{"name":"Traditional Medicine and History of Medicine Department of Persian Medicine School of Medicine Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Stem Cells Technology Research Center Shiraz University of Medical Sciences  Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Moghadam","family":"Farid","sequence":"additional","affiliation":[{"name":"Endocrinology and Metabolism Research Center Endocrinology and Metabolism Clinical Sciences Institute Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadreza","family":"Eftekharian","sequence":"additional","affiliation":[{"name":"Student Research Committee Jahrom University of Medical Sciences  Jahrom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6684-373X","authenticated-orcid":false,"given":"Paniz","family":"Naseri","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry Faculty of Pharmacy Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8007-2543","authenticated-orcid":false,"given":"Mohammad","family":"Mahdavi","sequence":"additional","affiliation":[{"name":"Endocrinology and Metabolism Research Center Endocrinology and Metabolism Clinical Sciences Institute Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-8058","authenticated-orcid":false,"given":"Mina","family":"Saeedi","sequence":"additional","affiliation":[{"name":"Medicinal Plants Research Center Faculty of Pharmacy Tehran University of Medical Sciences  Tehran Iran"},{"name":"Persian Medicine and Pharmacy Research Center Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahmineh","family":"Akbarzadeh","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry Faculty of Pharmacy Tehran University of Medical Sciences  Tehran Iran"},{"name":"Persian Medicine and Pharmacy Research Center Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"2254","DOI":"10.1016\/j.cell.2025.12.049","article-title":"Hallmarks of Cancer\u2014Then and now, and Beyond","volume":"89","author":"Hanahan D.","year":"2026","journal-title":"Cell"},{"key":"e_1_2_9_3_1","first-page":"85","article-title":"Global Burden of Cancer","volume":"79","author":"Ma X.","year":"2006","journal-title":"Yale Journal of Biology and Medicine"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","DOI":"10.1259\/bjr.20230334","article-title":"The Use of Radiotherapy, Surgery and Chemotherapy in the Curative Treatment of Cancer: Results From the FORTY (Favourable Outcomes From RadioTherapY) 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