{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:51:30Z","timestamp":1791312690648,"version":"4.3.1"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The study investigated how solvent choice affects the extraction of bioactive phytochemicals from\n                    <jats:italic>Stachys pinardii<\/jats:italic>\n                    . Methanol and aqueous extracts of aerial parts were obtained using ultrasound\u2010assisted extraction and analyzed for phytochemical content, antioxidant capacity, and enzyme inhibition. The methanol extract showed higher total phenolic (52.56\u00a0mg GAEs\/g) and flavonoid contents (18.19\u00a0mg REs\/g) than the aqueous extract (15.62\u00a0mg GAEs\/g and 8.25\u00a0mg REs\/g). Liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry (LC\u2013ESI\u2013MS\/MS) analysis identified verbascoside and chlorogenic acid as major compounds in methanol, while aqueous extract was richer in caffeic acid and hydroxycinnamic derivatives. Despite lower phenolic content, the aqueous extract demonstrated stronger antioxidant activity across 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl (DPPH), 2,2\u2032\u2010azinobis\u2010(3\u2010ethylbenzothiazoline\u20106\u2010sulfonic acid) (ABTS), cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays, with lower IC\n                    <jats:sub>50<\/jats:sub>\n                    \/EC\n                    <jats:sub>50<\/jats:sub>\n                    values. It also exhibited superior enzyme inhibitory effects, particularly against \u03b1\u2010amylase (2.52\u00a0mg\/mL) and \u03b1\u2010glucosidase (1.58\u00a0mg\/mL), although its inhibitory activity remained lower than that of the standard acarbose. Overall, solvent polarity significantly influences both phytochemical composition and biological activity. The aqueous extract, despite its lower total quantified phenolic content, shows promising multifunctional bioactivity. Further studies should focus on bioactivity\u2010guided fractionation and mechanistic insights into individual compounds.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71812","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:11:03Z","timestamp":1791310263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Characterization and Biological Activity of\n                    <i>Stachys pinardii<\/i>\n                    Extracts"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7245-7363","authenticated-orcid":false,"given":"Zeyneb","family":"Karakus","sequence":"first","affiliation":[{"name":"Chemical Technology Program \u00c7ay Vocational School Afyon Kocatepe University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5094-2520","authenticated-orcid":false,"given":"Cengiz","family":"Sarikurkcu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Afyonkarahisar Health Sciences University  Afyonkarahisar T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8290-9880","authenticated-orcid":false,"given":"Arzuhan","family":"Sihoglu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services Vocational High School of Health Services Kilis 7 Aralik University  Kilis T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"issue":"2","key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.59566\/IJBS.2008.4089","article-title":"Free Radicals, Antioxidants in Disease and Health","volume":"4","author":"Pham\u2010Huy L. A.","year":"2008","journal-title":"International Journal of Biomedical Science"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973\u20107847.70902"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010021\u201000233\u20101"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010020\u201000114\u2010z"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines7100063"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29225345"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360\u20101385(97)01018\u20102"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15107313"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10600\u2010006\u20100070\u20101"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/0972060X.2023.2293960"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2011.04.013"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111639"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.12.012"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.10.078"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants10061054"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.21597\/jist.1309555"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.17798\/bitlisfen.1002210"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b04739"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf030723c"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1888693"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eujim.2020.101134"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2020.101711"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2020.112089"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.11.009"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2026.2620803"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2025.2486414"},{"issue":"1","key":"e_1_2_12_28_1","first-page":"81","article-title":"Tyrosinase, Acetyl\u2010and Butyryl\u2010cholinesterase Inhibitory Activity of Stachys lavandulifolia Vahl (Lamiaceae) and Its Major Constituents","volume":"9","author":"Tundis R.","year":"2015","journal-title":"Records of Natural Products"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15070785"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24213880"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpp\/rgae138"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202504699"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2015.10.016"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.21448\/ijsm.1721228"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111878"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmy003"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.11.003"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068\u2010016\u20100120\u20109"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/09637480600798132"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.09.001"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202503665"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules31081240"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126930"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2016.1168837"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules31050840"}],"container-title":["Chemistry &amp; Biodiversity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71812","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbdv.71812","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbdv.71812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:11:04Z","timestamp":1791310264000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbdv.71812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/cbdv.71812"],"URL":"https:\/\/doi.org\/10.1002\/cbdv.71812","archive":["Portico"],"relation":{},"ISSN":["1612-1872","1612-1880"],"issn-type":[{"value":"1612-1872","type":"print"},{"value":"1612-1880","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71812"}}