{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:45:51Z","timestamp":1791873951268,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The growing incidence of bacterial resistance, especially in gram\u2010positive infections, emphasizes how urgently new antibacterial medicines must be developed. This study presents the rational design and synthesis of a series of fused imidazo[1,2\u2010b]pyrazole\u20131,2,4\u2010oxadiazole\u20131,2,3\u2010triazole hybrids (\n                    <jats:bold>8a\u20138n<\/jats:bold>\n                    ) employing a multi\u2010step synthetic approach that includes oxidative cyclisation, amidoxime formation, Vilsmeier\u2010mediated cyclodehydration, and Cu(I)\u2010catalyzed azide\u2013alkyne cycloaddition (CuAAC). The synthesized compounds were assessed for their in vitro antibacterial efficacy against\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , and\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    . Multiple derivatives displayed notable antibacterial activity, with compounds\n                    <jats:bold>8j<\/jats:bold>\n                    and\n                    <jats:bold>8l<\/jats:bold>\n                    identified as the most effective (MIC\u00a0=\u00a01.56\u00a0\u00b5g\/mL), exhibiting antibacterial efficacy comparable to or surpassing that of the reference medication dicloxacillin. Biofilm inhibition tests further indicated that compounds\n                    <jats:bold>8j<\/jats:bold>\n                    and\n                    <jats:bold>8l<\/jats:bold>\n                    exhibit substantial antibiofilm action. Molecular docking analyses targeting penicillin\u2010binding protein (PDB ID:\n                    <jats:bold>1MWT<\/jats:bold>\n                    ) demonstrated advantageous binding affinities, with compound 8k displaying the most favorable binding score (\u22129.10\u00a0kcal\/mol), corroborating the experimental results. Structure\u2013activity relationship (SAR) research revealed that the inclusion of electron\u2010withdrawing substituents, especially dihalogen groups, markedly increases antibacterial efficacy. These results underscore the therapeutic potential of fused imidazo[1,2\u2010b]pyrazole\u20131,2,4\u2010oxadiazole\u20131,2,3\u2010triazole hybrids as effective antibacterial and antibiofilm agents against gram\u2010positive bacterial infections.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71816","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:55:21Z","timestamp":1791870921000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Imidazo[1,2\u2010b]Pyrazole\u20101,2,4\u2010oxadiazole Linked 1,2,3\u2010Triazoles: Synthesis, Antibacterial, Biofilm, and In Silico Studies"],"prefix":"10.1002","volume":"23","author":[{"given":"Suresh Kumar","family":"Murahari","sequence":"first","affiliation":[{"name":"Department of Chemistry Chaitanya (Deemed to Be University)  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5808-708X","authenticated-orcid":false,"given":"Kavitha","family":"Siddoju","sequence":"additional","affiliation":[{"name":"Department of Chemistry Chaitanya (Deemed to Be University)  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s42452-025-06730-x","article-title":"In Vitro Antibacterial Activity of Selected Plant Extracts Against Escherichia coli and Staphylococcus aureus Bacterial Strains","volume":"7","author":"Ibn Awadh H.","year":"2025","journal-title":"Discover Applied Sciences"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.25122\/jml\u20102023\u20100404"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins12040223"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15143258"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/533590"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1444\u20100903.2005.00976.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2017.05.014"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2019.02.055"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.06.051"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030\u2010011\u20109319\u20100"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2017.01.010"},{"key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1086\/367933","article-title":"Voriconazole: A New Triazole Antifungal Agent","volume":"36","author":"Saravolatz L. 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