{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:45:57Z","timestamp":1791873957038,"version":"4.3.4"},"reference-count":93,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The brewing industry generates large amounts of waste containing bioactive compounds with potential applications in the pharmaceutical and cosmetic industries. This study optimized the extraction of bioactive compounds from hot trub using 70:30 (v\/v) ethanol:water as the extraction solvent, with time and temperature optimized through an experimental design. The optimized extract exhibited high antioxidant activity and was rich in phenolic acids, flavonoids, and xanthohumol. The extract demonstrated promising photoprotective properties, with a sun protection factor of 13.84, suggesting its potential for dermocosmetic use. The extract was encapsulated in zein nanoparticles, including chitosan\u2010coated and sodium caseinate\u2010coated variants, to enhance stability, bioavailability, and bioactivity. The effect of the encapsulated extract on cell viability was evaluated against the MCF\u20107 breast cancer cell line. Results indicated that encapsulation in zein nanoparticles improved the in vitro effect of the extract on MCF\u20107 cell viability, with chitosan and sodium caseinate coatings enhancing this effect. These findings highlight hot trub as a sustainable source of bioactive compounds with potential for dermocosmetic applications and as a basis for further investigation in cancer research. Future studies should focus on evaluating the long\u2010term stability of the nanoparticles and on assessing their biological activity in more complex models.<\/jats:p>","DOI":"10.1002\/cbdv.71827","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:55:23Z","timestamp":1791870923000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Valorization of Hot Trub: Extraction and Nanoparticle Encapsulation for Antitumor Applications"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8250-8064","authenticated-orcid":false,"given":"Lilian Tatiani Dusman","family":"Tonin","sequence":"first","affiliation":[{"name":"Postgraduate Program in Chemical Engineering Federal Technological University of Paran\u00e1 (UTFPR)  Apucarana Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella Salvat","family":"Luccas","sequence":"additional","affiliation":[{"name":"Chemical Engineering Course Coordination Federal Technological University of Paran\u00e1 (UTFPR)  Apucarana Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Barbosa","family":"da Silva","sequence":"additional","affiliation":[{"name":"Chemical Engineering Course Coordination Federal Technological University of Paran\u00e1 (UTFPR)  Apucarana Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna Martins","family":"Domingos","sequence":"additional","affiliation":[{"name":"Chemical Engineering Course Coordination Federal Technological University of Paran\u00e1 (UTFPR)  Apucarana Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8699-2033","authenticated-orcid":false,"given":"Grazielle","family":"Jesus dos Santos","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Chemical Engineering Federal Technological University of Paran\u00e1 (UTFPR)  Apucarana Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4483-5638","authenticated-orcid":false,"given":"Elis\u00e2ngela","family":"D\u00fcsman","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Federal Technological University of Paran\u00e1 (UTFPR)  Francisco Beltr\u00e3o Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Beatriz Sousa","family":"Santana","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Federal Technological University of Paran\u00e1 (UTFPR)  Francisco Beltr\u00e3o Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia Aline","family":"Bressiani","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Federal Technological University of Paran\u00e1 (UTFPR)  Francisco Beltr\u00e3o Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1gatha Giovanna","family":"Fragoso","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biology Federal Technological University of Paran\u00e1 (UTFPR)  Francisco Beltr\u00e3o Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica Priscila Parussolo","family":"Tonin","sequence":"additional","affiliation":[{"name":"Laboratory Fenn of Mass Spectrometry Department of Chemistry Federal University of Paran\u00e1 (UFPR)  Jandaia do Sul Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4835-7773","authenticated-orcid":false,"given":"Eduardo C\u00e9sar","family":"Meurer","sequence":"additional","affiliation":[{"name":"Laboratory Fenn of Mass Spectrometry Department of Chemistry Federal University of Paran\u00e1 (UFPR)  Jandaia do Sul Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s12291-014-0446-0","article-title":"Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases","volume":"30","author":"Phaniendra A.","year":"2015","journal-title":"Indian Journal of Clinical Biochemistry"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1007\/s12649-017-9939-4","article-title":"Biological Properties of Polyphenols Extracts From Agro Industry's Wastes","volume":"9","author":"Di Donato P.","year":"2018","journal-title":"Waste and Biomass Valorization"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1055\/s-0042-110180","article-title":"In Vitro Dermocosmetic Evaluation of Bark Extracts From Common Temperate 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