{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T07:34:47Z","timestamp":1793172887096,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Four new benzimidazolium\u2013chalcone salt derivatives were synthesized and successfully characterized using\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>13<\/jats:sup>\n                    C NMR, and FT\u2010IR spectroscopy. Their antioxidant activity, acetylcholinesterase (AChE) inhibitory potential, antibacterial and antibiofilm activities against MRSA and MSSA strains, as well as their anticancer and apoptosis\u2010inducing properties in human colon adenocarcinoma (HT\u201029) cells, were comprehensively evaluated. Among the synthesized compounds,\n                    <jats:bold>3a<\/jats:bold>\n                    exhibited the strongest antioxidant activity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 10.13\u00a0\u00b1\u00a00.30\u00a0\u00b5g\/mL in the ABTS assay and 79.40\u00a0\u00b5g\/mL in the DPPH assay, and demonstrated the highest AChE inhibitory effect. Molecular docking analysis further supported these findings, revealing a favorable binding affinity of compound\n                    <jats:bold>3a<\/jats:bold>\n                    toward the 4EY7 target protein, with a docking score of \u22128.619\u00a0kcal\/mol. Antibacterial evaluation using the agar well diffusion method showed that compound\n                    <jats:bold>3b<\/jats:bold>\n                    produced the largest inhibition zone (19.2\u00a0\u00b1\u00a00.6\u00a0mm). Consistently, MIC analysis confirmed that\n                    <jats:bold>3b<\/jats:bold>\n                    displayed potent antibacterial activity against MRSA (MIC: 1\u00a0\u00b5g\/mL) and exhibited the most pronounced antibiofilm effect among the tested derivatives.\n                  <\/jats:p>\n                  <jats:p>\n                    In cytotoxicity assays, compound\n                    <jats:bold>3c<\/jats:bold>\n                    demonstrated the highest anticancer activity against HT\u201029 cells, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 16.7\u00a0\u00b1\u00a02.09\u00a0\u00b5M. Apoptosis analysis revealed that\n                    <jats:bold>3c<\/jats:bold>\n                    predominantly induced early\u2010stage apoptosis.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71828","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:33:38Z","timestamp":1792064018000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Biological Evaluation of Benzimidazolium\u2013Chalcone Derivatives: Antioxidant, Anti\u2010AChE, Anticancer, and Anti\u2010MRSA Activities With Docking Insights"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3563-2468","authenticated-orcid":false,"given":"Mehmet","family":"\u00c7imentepe","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy Harran University  Sanliurfa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4858-4039","authenticated-orcid":false,"given":"Hakan","family":"\u00dcnver","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Eskisehir Technical University  Eskisehir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2092-7829","authenticated-orcid":false,"given":"Adem","family":"Necip","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Services, Vocational School of Health Services Harran University  Sanliurfa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1077-0626","authenticated-orcid":false,"given":"Mehmet","family":"Ersatir","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Art and Science Cukurova University  Adana T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1837-6754","authenticated-orcid":false,"given":"Burcu","family":"Demirba\u011f","sequence":"additional","affiliation":[{"name":"Health Services Vocational School Medical Laboratory Techniques Program Mersin University  Mersin T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zge \u00d6zt\u00fcrk","family":"\u00c7imentepe","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy Harran University  Sanliurfa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1346-312X","authenticated-orcid":false,"given":"Metin","family":"Yildirim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Cukurova University  Adana 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