{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T07:47:35Z","timestamp":1791791255357,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry &amp;amp; Biodiversity"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acetylcholinesterase (AChE) and urease are critical enzymes involved in various pathological conditions associated with cellular and tissue damage. Natural products are increasingly explored as safer alternatives to synthetic inhibitors. This study evaluates the phytochemical composition and enzyme inhibitory potential of\n                    <jats:italic>Pistacia lentiscus<\/jats:italic>\n                    leaves using a green extraction approach. Bioactive compounds were extracted via lactic acid\u2010assisted ultrasound, and the crude extract was characterized for total phenolics, flavonoids, LC\u2010MS profile, and in vitro inhibition of acetylcholinesterase and urease. Molecular docking was performed to investigate ligand\u2013enzyme interactions. The extract contained 142.13\u00a0\u00b1\u00a00.06\u00a0mg GAE\/g DW of polyphenols and 16.17\u00a0\u00b1\u00a00.02\u00a0mg QE\/g DW of flavonoids. LC\u2010MS analysis identified phenolic acids, phenolic glycosides, coumarins, and multiple flavonoid subclasses, including flavonols, flavanols, flavanones, flavones, and flavonolignans. The extract exhibited significant inhibition of both enzymes. Docking studies revealed key interactions, such as hydrogen bonds, \u03c0\u2010alkyl, and \u03c0\u2010sigma, within the active sites, with hesperidin and verbascoside showing the strongest binding affinities (acetylcholinesterase: \u201310.1 and \u201310.2\u00a0kcal\/mol; urease: \u20139.6 and \u20139.2\u00a0kcal\/mol, respectively). These results demonstrate that ultrasound\u2010assisted lactic acid extraction efficiently isolates phenolic compounds from\n                    <jats:italic>P. lentiscus<\/jats:italic>\n                    , and the identified metabolites may serve as promising templates for the development of potent enzyme inhibitors.\n                  <\/jats:p>","DOI":"10.1002\/cbdv.71831","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T07:11:48Z","timestamp":1791789108000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Green Extraction of Phenolic Compounds From\n                    <i>Pistacia lentiscus<\/i>\n                    Leaves Coupled With LC\u2010MS Characterization and Structure\u2010Based Enzyme Inhibition Analysis"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7368-9054","authenticated-orcid":false,"given":"Sidali","family":"Zaidi","sequence":"first","affiliation":[{"name":"Centre de Recherche en Technologies Agro\u2010Alimentaires Campus Universitaire  Bejaia Algeria"},{"name":"Facult\u00e9 Des Sciences de La Nature Et de La Vie, Laboratoire de Biochimie Appliqu\u00e9e Universit\u00e9 de Bejaia  Bejaia Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naima","family":"Saidene","sequence":"additional","affiliation":[{"name":"Facult\u00e9 Des Sciences de La Nature Et de La Vie, Laboratoire de Biochimie Appliqu\u00e9e Universit\u00e9 de Bejaia  Bejaia Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radia","family":"Kadi","sequence":"additional","affiliation":[{"name":"Facult\u00e9 Des Sciences de La Nature Et de La Vie Laboratoire de Biomath\u00e9matique, Biophysique, Biochimie Et Scientom\u00e9trie (3BS) Universit\u00e9 de Bejaia  Bejaia Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadjet","family":"Benaida\u2010Debbache","sequence":"additional","affiliation":[{"name":"Facult\u00e9 Des Sciences de La Nature Et de La Vie, Laboratoire de Biochimie Appliqu\u00e9e Universit\u00e9 de Bejaia  Bejaia Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694\u2010017\u20109594\u20102"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/162750"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2016.03.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2018.03.012"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2024.101678"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2023.08.063"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10101579"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2005.04.028"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1088\/1742\u20106596\/1869\/1\/012055"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2478\/asn\u20102020\u20100005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20102952(61)90145\u20109"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1021\/ac60252a045","article-title":"Phenol\u2010Hypochlorite 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