{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:06:35Z","timestamp":1760141195429,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,5,30]],"date-time":"2003-05-30T00:00:00Z","timestamp":1054252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When plasma proteins leak from circulation into the renal tubular lumen in the proteinuric renal diseases, nephrotoxicity of filtered albumin (and\/or molecules bound to it) may be important in the subsequent development of tubulo\u2010interstitial damage which contributes to the progression of the disease. When cultured opossum kidney (OK) proximal tubular cells were exposed to bovine serum albumin for 3 days <jats:italic>in vitro<\/jats:italic>, increased cell division ([<jats:sup>3<\/jats:sup>H]\u2010thymidine incorporation) and cellular hypertrophy (increased protein\/DNA ratio) were observed. Both effects were halved if defatted albumin was used. A trivial explanation for the growth responses is that free fatty acids carried on the albumin, and amino acids generated by intracellular degradation of the albumin, are exerting a non\u2010specific growth effect as metabolic fuels which are oxidized to generate ATP. However, the water\u2010soluble free fatty acid octanoate (1 mmol\u2009l<jats:sup>\u22121<\/jats:sup>) had no significant effect on protein\/DNA ratio and a very variable stimulatory effect on [<jats:sup>3<\/jats:sup>H]\u2010thymidine incorporation, whereas an essential amino acid mixture or 1\u2009mmol\/l<jats:sup>\u22121<\/jats:sup> <jats:sc>l<\/jats:sc>\u2010Ala or <jats:sc>l<\/jats:sc>\u2010Phe only increased the protein\/DNA ratio. Furthermore no carnitine was added to the culture medium. This absence would have impaired mitochondrial transport (and hence oxidation) of long\u2010chain free fatty acids derived from the albumin. <jats:sc>l<\/jats:sc>\u2010Phe is also a poor substrate for mitochondrial oxidation in kidney. It is therefore concluded that the growth effects of albumin in OK proximal tubular cells are specific effects of the albumin protein and of the free fatty acids and amino acids derived from it, and not a non\u2010specific effect on metabolic fuel supply. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1045","type":"journal-article","created":{"date-parts":[[2003,12,22]],"date-time":"2003-12-22T03:03:24Z","timestamp":1072062204000},"page":"1-7","source":"Crossref","is-referenced-by-count":2,"title":["Free amino acids mimic the anabolic but not the proliferative effect of albumin in OK proximal tubular 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