{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:07:43Z","timestamp":1762916863890,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,7,15]],"date-time":"2003-07-15T00:00:00Z","timestamp":1058227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cathepsin K is a member of the papain superfamily of cysteine proteases and plays a pivotal role in osteoclast\u2010mediated bone resorption. This enzyme is an excellent target for antiresorptive therapies for osteopenic disorders such as osteoporosis.<jats:sup>1<\/jats:sup> Although isolated inhibitor studies on purified enzymes is required to discover potent and selective inhibitors of cathepsin K, a quantitative cytochemical assay<jats:sup>2<\/jats:sup> for cathepsin K would allow inhibitors to be tested on actual osteoclasts within sections of bone. Furthermore cathepsin K activity could be used to identify and analyse osteoclasts at definitive stages of their lifespan. A cytochemical assay is described that localizes osteoclast cathepsin K activity in unfixed, undecalcified cryostat sections of animal and human bone. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1078","type":"journal-article","created":{"date-parts":[[2003,8,5]],"date-time":"2003-08-05T11:47:32Z","timestamp":1060084052000},"page":"231-234","source":"Crossref","is-referenced-by-count":3,"title":["A cytochemical assay for osteoclast cathepsin K activity"],"prefix":"10.1002","volume":"21","author":[{"given":"Robert A.","family":"Dodds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,7,15]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_1_2_1","DOI":"10.2174\/1381612003401569"},{"doi-asserted-by":"publisher","key":"e_1_2_1_3_1","DOI":"10.1126\/science.2528207"},{"doi-asserted-by":"publisher","key":"e_1_2_1_4_1","DOI":"10.1083\/jcb.106.6.1863"},{"doi-asserted-by":"publisher","key":"e_1_2_1_5_1","DOI":"10.1083\/jcb.111.3.1305"},{"doi-asserted-by":"publisher","key":"e_1_2_1_6_1","DOI":"10.1007\/BF02263497"},{"doi-asserted-by":"publisher","key":"e_1_2_1_7_1","DOI":"10.1002\/jcb.240560116"},{"doi-asserted-by":"publisher","key":"e_1_2_1_8_1","DOI":"10.1007\/BF00298591"},{"doi-asserted-by":"publisher","key":"e_1_2_1_9_1","DOI":"10.1016\/0006-291X(84)90560-6"},{"doi-asserted-by":"publisher","key":"e_1_2_1_10_1","DOI":"10.1002\/jbmr.5650070411"},{"doi-asserted-by":"publisher","key":"e_1_2_1_11_1","DOI":"10.1016\/8756-3282(87)90007-X"},{"doi-asserted-by":"publisher","key":"e_1_2_1_12_1","DOI":"10.1074\/jbc.271.21.12511"},{"doi-asserted-by":"publisher","key":"e_1_2_1_13_1","DOI":"10.1016\/S0021-9258(17)42227-7"},{"doi-asserted-by":"publisher","key":"e_1_2_1_14_1","DOI":"10.1016\/0014-5793(94)01349-6"},{"doi-asserted-by":"publisher","key":"e_1_2_1_15_1","DOI":"10.1515\/bchm3.1995.376.6.379"},{"doi-asserted-by":"publisher","key":"e_1_2_1_16_1","DOI":"10.1002\/jbmr.5650100809"},{"doi-asserted-by":"publisher","key":"e_1_2_1_17_1","DOI":"10.1006\/bbrc.1995.1013"},{"key":"e_1_2_1_18_1","first-page":"586","article-title":"Immunolocalization of cathepsin K at sites of bone resorption","volume":"22","author":"Fukunishi S","year":"1997","journal-title":"J Orthop Res"},{"doi-asserted-by":"publisher","key":"e_1_2_1_19_1","DOI":"10.1074\/jbc.271.21.12517"},{"doi-asserted-by":"publisher","key":"e_1_2_1_20_1","DOI":"10.1074\/jbc.271.4.2126"},{"doi-asserted-by":"publisher","key":"e_1_2_1_21_1","DOI":"10.1074\/jbc.273.48.32347"},{"doi-asserted-by":"publisher","key":"e_1_2_1_22_1","DOI":"10.1007\/BF01106087"},{"doi-asserted-by":"publisher","key":"e_1_2_1_23_1","DOI":"10.1097\/00003086-197101000-00035"},{"doi-asserted-by":"publisher","key":"e_1_2_1_24_1","DOI":"10.1097\/00000441-197603000-00011"},{"doi-asserted-by":"publisher","key":"e_1_2_1_25_1","DOI":"10.2106\/00004623-197860080-00023"},{"doi-asserted-by":"publisher","key":"e_1_2_1_26_1","DOI":"10.1016\/0002-9343(66)90108-2"},{"doi-asserted-by":"publisher","key":"e_1_2_1_27_1","DOI":"10.1126\/science.273.5279.1236"},{"doi-asserted-by":"publisher","key":"e_1_2_1_28_1","DOI":"10.1101\/gr.6.11.1050"},{"doi-asserted-by":"publisher","key":"e_1_2_1_29_1","DOI":"10.1073\/pnas.95.23.13453"},{"doi-asserted-by":"publisher","key":"e_1_2_1_30_1","DOI":"10.1359\/jbmr.1999.14.10.1654"},{"doi-asserted-by":"publisher","key":"e_1_2_1_31_1","DOI":"10.1359\/jbmr.1997.12.9.1396"},{"doi-asserted-by":"publisher","key":"e_1_2_1_32_1","DOI":"10.1073\/pnas.94.26.14249"},{"doi-asserted-by":"publisher","key":"e_1_2_1_33_1","DOI":"10.1248\/bpb.19.1026"},{"doi-asserted-by":"publisher","key":"e_1_2_1_34_1","DOI":"10.1177\/37.5.2703699"},{"volume-title":"Practical Histochemistry","author":"Chayen J","key":"e_1_2_1_35_1"},{"doi-asserted-by":"publisher","key":"e_1_2_1_36_1","DOI":"10.1177\/42.5.8157931"},{"doi-asserted-by":"publisher","key":"e_1_2_1_37_1","DOI":"10.1359\/jbmr.2001.16.3.478"},{"doi-asserted-by":"publisher","key":"e_1_2_1_38_1","DOI":"10.1016\/S8756-3282(02)00675-0"},{"doi-asserted-by":"publisher","key":"e_1_2_1_39_1","DOI":"10.1074\/jbc.M010684200"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.1078","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.1078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:51:29Z","timestamp":1760039489000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.1078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7,15]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1002\/cbf.1078"],"URL":"https:\/\/doi.org\/10.1002\/cbf.1078","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[2003,7,15]]}}}