{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:47:07Z","timestamp":1760060827789,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,7]],"date-time":"2004-10-07T00:00:00Z","timestamp":1097107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The flavin monooxygenases (FMO) catalyse the NADPH and oxygen\u2010dependent oxidation of a wide range of nucleophilic nitrogen\u2010, sulfur\u2010, phosphorus\u2010, and selenium heteroatom\u2010containing chemicals, drugs, and agricultural agents. In the present study, sheep liver microsomal FMO activity was determined by measuring the S\u2010oxidation rate of methimazole and the average specific activity obtained from different microsomal preparations was found to be 3.8\u2009\u00b1\u20091.5\u2009nmol methimazole oxidized min<jats:sup>\u22121<\/jats:sup>\u2009mg<jats:sup>\u22121<\/jats:sup> microsomal protein (mean\u2009\u00b1\u2009SE, <jats:italic>n<\/jats:italic>\u2009=\u20097). The presence of 0.1% Triton X\u2010100 in the reaction mixture caused an increase of specific sheep liver microsomal FMO activity towards methimazole to 6.1\u2009\u00b1\u20091.4\u2009nmol methimazole oxidized min<jats:sup>\u22121<\/jats:sup>\u2009mg<jats:sup>\u22121<\/jats:sup> microsomal protein (mean\u2009\u00b1\u2009SE, <jats:italic>n<\/jats:italic>\u2009=\u20096). Metabolism of imipramine and chlorpromazine was measured by following the oxidation of cofactor NADPH spectrophotometrically at 340\u2009nm. Sheep liver microsomal FMO activity towards imipramine and chlorpromazine was found to be 10.7 and 12.3\u2009nmol NADPH oxidized min<jats:sup>\u22121<\/jats:sup>\u2009mg<jats:sup>\u22121<\/jats:sup> microsomal protein, respectively. Characterization of sheep liver enzyme was carried out using methimazole as substrate and the maximum FMO enzyme activity was detected at 37\u00b0C and at pH 8.0. The apparent <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> value of sheep liver microsomal FMO for methimazole was 0.118\u2009m<jats:sc>M<\/jats:sc>. Effects of the detergents Triton X\u2010100, Cholate, and Emulgen 913, on FMO activity were determined and FMO activity was found to increase with the addition of detergents to the reaction medium. Sheep liver microsomal FMO\u2010catalysed methimazole oxidation was inhibited by imipramine and chlorpromazine when these drugs were used at high concentrations. Western blot\u2013immunochemical analysis revealed the presence of FMO3 in sheep liver microsomes. 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