{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:50:07Z","timestamp":1760061007367,"version":"build-2065373602"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,9,5]],"date-time":"2005-09-05T00:00:00Z","timestamp":1125878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of high voltage\u2010activated calcium channels in the rat pineal gland is well known. However, their role in pineal metabolism is not completely understood and is even controversial. Better to understand this matter, we investigated the effects of L\u2010, N\u2010 or P\/Q\u2010type calcium channel blockers (nifedipine, \u03c9\u2010conotoxin GVIA, \u03c9\u2010agatoxin IVA, respectively) on melatonin content and arylalkylamine\u2010N\u2010acetyltransferase activity of denervated rat pineal glands kept for 48\u2009h in culture and stimulated with norepinephrine. Melatonin was measured by high performance liquid chromatography with electrochemical detection and arylalkylamine\u2010N\u2010acetyltransferase activity was quantified by radiometric assay. Pre\u2010incubation with any of these high voltage\u2010activated calcium channel blockers reduced the melatonin production induced by norepinephrine although arylalkylamine\u2010N\u2010acetyltransferase activity was reduced only by the N\u2010type calcium channel antagonist, \u03c9\u2010conotoxin GVIA. The results indicate that calcium influx through L\u2010, N\u2010 or P\/Q\u2010type of high voltage\u2010activated calcium channels is necessary for the full expression of the metabolic process leading to melatonin synthesis in the rat pineal glands. However, the mechanisms involved in this process are different for the L\u2010 or P\/Q\u2010 and N\u2010type calcium channels. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1270","type":"journal-article","created":{"date-parts":[[2005,9,5]],"date-time":"2005-09-05T08:47:10Z","timestamp":1125910030000},"page":"499-505","source":"Crossref","is-referenced-by-count":14,"title":["Effects of the blockade of high voltage\u2010activated calcium channels on <i>in vitro<\/i> pineal melatonin synthesis"],"prefix":"10.1002","volume":"24","author":[{"given":"Solange C.","family":"Afeche","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roseli","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julieta H.","family":"Scialfa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilza M.","family":"Terra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio 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