{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:10:30Z","timestamp":1762521030497,"version":"build-2065373602"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,1,6]],"date-time":"2006-01-06T00:00:00Z","timestamp":1136505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Biochemistry &amp;amp; Function"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>[Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations were found in human osteoblast\u2010like cells (hOB cells) exposed to high\u2010lipid bovine serum albumin (BSA), but not when exposed to low\u2010lipid BSA. These [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations were inhibited by heptanol and suramin, which implies that gap junctions and purinergic signalling may be important for these [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations. The high\u2010lipid BSA preparation that was used contains arachidonic acid. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations could be induced by low lipid albumin with arachidonic acid added. The albumin\u2010bound lipids were also important for osteoblast growth since DNA synthesis and the total cell protein content was higher in hOB cells exposed to high\u2010lipid BSA. The effect of arachidonic acid on hOB cell proliferation was bone\u2010donor dependent; both stimulatory and inhibitory effects were observed. The physiological importance of albumin\u2010bound lipids is unclear; given that albumin has only minimal contact with osteoblasts under normal conditions. Only when bone capillaries are disrupted, e.g. during a fracture, would significant amounts of albumin reach osteoblasts. Albumin\u2010bound lipids could therefore contribute to stimulation of osteoblast proliferation during fracture healing. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/cbf.1316","type":"journal-article","created":{"date-parts":[[2006,1,6]],"date-time":"2006-01-06T06:16:23Z","timestamp":1136528183000},"page":"245-249","source":"Crossref","is-referenced-by-count":13,"title":["Albumin\u2010bound lipids induce free cytoplasmic calcium oscillations in human osteoblast\u2010like cells"],"prefix":"10.1002","volume":"25","author":[{"given":"Jon A.","family":"Tsai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Lagumdzija","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Stark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"Kindmark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1339"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1426"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199704)19:4<343::AID-GLIA7>3.0.CO;2-Y"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110917"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01351839"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/127.6.1084"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900425"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370517"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90240-1"},{"key":"e_1_2_1_11_2","first-page":"26","article-title":"A novel lipid\u2010rich albumin supplement for reduced\u2010serum and serum\u2010free cell culture","volume":"13","author":"Airey P","year":"1991","journal-title":"Focus (product information from Life Technologies)"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02556563"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00805-0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.497"}],"container-title":["Cell Biochemistry and Function"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbf.1316","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbf.1316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:01:09Z","timestamp":1760036469000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/cbf.1316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,6]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1002\/cbf.1316"],"URL":"https:\/\/doi.org\/10.1002\/cbf.1316","archive":["Portico"],"relation":{},"ISSN":["0263-6484","1099-0844"],"issn-type":[{"type":"print","value":"0263-6484"},{"type":"electronic","value":"1099-0844"}],"subject":[],"published":{"date-parts":[[2006,1,6]]}}}